Detailed information NTG0622425

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LL046_RS05230
• Genome accession: NZ_CP086088
• Coordinates: 1021484..1022806 (-)
• Length: 440 a.a.
• NCBI ID: WP_012897706.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain EIP13A
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1016484..1027806

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL046_RS05200 (LL046_05180)	-	1017627..1018595 (+)	969	WP_003130649.1	PhoH family protein	-
LL046_RS05205 (LL046_05185)	-	1018653..1019132 (+)	480	WP_003130650.1	NUDIX hydrolase	-
LL046_RS05210 (LL046_05190)	ybeY	1019284..1019772 (+)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
LL046_RS05215 (LL046_05195)	-	1019756..1020211 (+)	456	WP_003130653.1	diacylglycerol kinase family protein	-
LL046_RS05220 (LL046_05200)	-	1020225..1020803 (+)	579	WP_012897704.1	nucleotidyltransferase family protein	-
LL046_RS05225 (LL046_05205)	comFC	1020837..1021241 (-)	405	WP_240819059.1	ComF family protein	Machinery gene
LL046_RS05230 (LL046_05210)	comFA	1021484..1022806 (-)	1323	WP_012897706.1	DEAD/DEAH box helicase	Machinery gene
LL046_RS05235 (LL046_05215)	-	1022863..1023492 (+)	630	WP_003130660.1	YigZ family protein	-
LL046_RS05240 (LL046_05220)	-	1023626..1024123 (+)	498	WP_012897708.1	VanZ family protein	-
LL046_RS05245 (LL046_05225)	-	1024171..1025190 (+)	1020	WP_012897709.1	DUF475 domain-containing protein	-
LL046_RS05250 (LL046_05230)	-	1025300..1026052 (+)	753	WP_012897710.1	bifunctional metallophosphatase/5'-nucleotidase	-
LL046_RS05255 (LL046_05235)	glyQ	1026338..1027291 (+)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50205.70 Da
• Isoelectric Point: 10.1065

>NTDB_id=622425 LL046_RS05230 WP_012897706.1 1021484..1022806(-) (comFA) [Lactococcus lactis subsp. lactis strain EIP13A]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVLYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSSERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=622425 LL046_RS05230 WP_012897706.1 1021484..1022806(-) (comFA) [Lactococcus lactis subsp. lactis strain EIP13A]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTACTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATCATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTTCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (300-404)

• Type III restriction enzyme, res subunit (ResIII) (114-233)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.25	90.909	0.675
comFA/cflA	Streptococcus mitis SK321	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae Rx1	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae D39	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae R6	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae TIGR4	52.01	90.455	0.47
comFA/cflA	Streptococcus mitis NCTC 12261	51.256	90.455	0.464
comFA	Bacillus subtilis subsp. subtilis str. 168	37.029	100	0.38