Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   M5T46_RS03970 Genome accession   NZ_CP103479
Coordinates   815140..815799 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 4621     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 810140..820799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T46_RS03940 (M5T46_03940) ygiN 811041..811355 (-) 315 WP_000958604.1 putative quinol monooxygenase -
  M5T46_RS03945 (M5T46_03945) mdaB 811386..811967 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  M5T46_RS03950 (M5T46_03950) - 812217..812696 (+) 480 WP_000065334.1 Hcp family type VI secretion system effector -
  M5T46_RS03955 (M5T46_03955) - 812699..813409 (+) 711 WP_000834027.1 hypothetical protein -
  M5T46_RS03960 (M5T46_03960) ygiZ 813416..813745 (+) 330 WP_000914694.1 DUF2645 family protein -
  M5T46_RS03965 (M5T46_03965) qseC 813794..815143 (-) 1350 WP_000673373.1 quorum sensing histidine kinase QseC -
  M5T46_RS03970 (M5T46_03970) ciaR 815140..815799 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  M5T46_RS03975 (M5T46_03975) ygiW 815951..816343 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  M5T46_RS03980 (M5T46_03980) ygiV 816396..816878 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  M5T46_RS03985 (M5T46_03985) ygiS 816987..818594 (+) 1608 WP_001327858.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=622364 M5T46_RS03970 WP_001221493.1 815140..815799(-) (ciaR) [Escherichia coli strain 4621]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=622364 M5T46_RS03970 WP_001221493.1 815140..815799(-) (ciaR) [Escherichia coli strain 4621]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365