Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LKW27_RS20090 Genome accession   NZ_CP086023
Coordinates   4425972..4427561 (-) Length   529 a.a.
NCBI ID   WP_011793602.1    Uniprot ID   A1TJ88
Organism   Paracidovorax citrulli strain HPP21-3-3B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4420972..4432561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LKW27_RS20070 (LKW27_20105) - 4421535..4422017 (-) 483 WP_017440101.1 putative toxin-antitoxin system toxin component, PIN family -
  LKW27_RS20075 (LKW27_20110) - 4422007..4423332 (-) 1326 WP_046060323.1 THUMP domain-containing protein -
  LKW27_RS20080 (LKW27_20115) - 4423488..4425344 (+) 1857 WP_046060322.1 methyl-accepting chemotaxis protein -
  LKW27_RS20085 (LKW27_20120) - 4425405..4425947 (+) 543 WP_046060446.1 TspO/MBR family protein -
  LKW27_RS20090 (LKW27_20125) rpoN/rpoN1 4425972..4427561 (-) 1590 WP_011793602.1 RNA polymerase factor sigma-54 Machinery gene
  LKW27_RS20095 (LKW27_20130) lptB 4427561..4428352 (-) 792 WP_046060321.1 LPS export ABC transporter ATP-binding protein -
  LKW27_RS20100 (LKW27_20135) lptA 4428355..4429005 (-) 651 WP_046060320.1 lipopolysaccharide transport periplasmic protein LptA -
  LKW27_RS20105 (LKW27_20140) - 4429125..4429781 (-) 657 WP_017439849.1 thiol:disulfide interchange protein DsbA/DsbL -
  LKW27_RS20110 (LKW27_20145) - 4429851..4430498 (-) 648 WP_046060319.1 SPOR domain-containing protein -
  LKW27_RS20115 (LKW27_20150) argS 4430504..4432213 (-) 1710 WP_017439551.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 57887.10 Da        Isoelectric Point: 4.6032

>NTDB_id=622115 LKW27_RS20090 WP_011793602.1 4425972..4427561(-) (rpoN/rpoN1) [Paracidovorax citrulli strain HPP21-3-3B]
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFGLLQADAPVSDADRGAEESLY
SPASGAVETAASAPGEGASEGSEAPEAPDAPDWEGDGTVEMAPDDGEWGGEAPARTRNQLGDEDDTDATELAREHESLTD
YLHRQALSLRLSEVDRAALRFLIESLNDDGYLDESLEALAVALAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
ECLALQLRALQEDGDAEPEVLQAALRICNQPLEMLARRDVRRLAQGCGAGEECTRAAMALIARLEPRPGRAFAQVERNII
VPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGALRGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAI
VDRQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=622115 LKW27_RS20090 WP_011793602.1 4425972..4427561(-) (rpoN/rpoN1) [Paracidovorax citrulli strain HPP21-3-3B]
ATGAAGCCGGGACTGTCGCTGCGCATCTCGCAGCATCTGGCGCTGACGCCGCAACTGCAGCAATCGATCCGGCTGCTGCA
GCTGTCCACGCTGGAGCTGTCGCAGGAAGTCGAGCAGATGCTCGACGAGAACCCCTTCCTGGAGCGCAACCCGGACGAGG
CACCGCGCGAGGAGTTCGGGCTGCTGCAGGCGGATGCGCCGGTGAGCGACGCCGACCGCGGGGCGGAGGAGTCCCTCTAT
TCGCCGGCCAGCGGGGCGGTGGAGACGGCGGCCTCGGCGCCGGGCGAGGGCGCATCCGAAGGATCGGAGGCGCCGGAAGC
TCCCGATGCGCCGGACTGGGAGGGCGACGGCACCGTCGAGATGGCGCCCGACGACGGCGAGTGGGGCGGCGAGGCGCCCG
CGCGCACGCGCAACCAGCTGGGGGACGAAGACGACACGGACGCGACCGAACTCGCGCGCGAGCACGAGTCGCTCACCGAT
TACCTGCACCGGCAGGCGCTGTCGCTGCGCCTGTCGGAGGTGGACCGCGCGGCGCTGCGCTTCCTGATCGAATCGCTGAA
CGACGACGGCTACCTCGACGAATCGCTGGAGGCACTGGCCGTGGCCCTCGCGGGCGAGGAAGACCCCGAGCAGGTGGAGG
AACTGGTGCATCGCTTCACGGTGGCGCTGCGGCTGCTGCAGAGCCTGGAGCCCGTGGGCGTGGGCGCGCGCGGCCTGAGC
GAATGCCTGGCGCTGCAGCTGCGGGCCCTGCAGGAAGATGGCGACGCGGAGCCCGAAGTGCTGCAGGCGGCGCTGCGCAT
CTGCAATCAGCCGCTGGAGATGCTGGCGCGGCGCGACGTGCGCCGGCTCGCGCAGGGTTGCGGTGCCGGCGAGGAGTGCA
CGCGCGCGGCGATGGCGCTCATCGCGCGGCTGGAGCCCCGGCCGGGCCGGGCTTTCGCGCAGGTCGAGCGCAACATCATC
GTGCCCGACGTGATCGTGAAGAAGAGCAGTGGCCGCGGGGCCGCGCATGCGTTCACCGTGCAGCTCAACCCGGATGTCAT
GCCGCGGCTGCGCGTGCACGACATCTATGCCGGGGCGCTGCGCGGCAACCGCGGCGGCGAGGGGCACCAGGCGCTGCAGC
AGCGGCTGCAGGAAGCGCGTTGGTTCATCAAGAACATCCAGCAGCGGTTCGACACCATCCTGCGCGTCTCGCGCGCCATC
GTGGACCGGCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGGCCGCTCGTGCTGCGCGACATCGCCGACGAGCT
GGGGCTGCACGAATCGACGATCAGCCGGGTGACCACGGCCAAGTACATGGCCACGCCCCAGGGTACCTACGAGCTGAAGT
ATTTCTTCGGCTCGGGCCTGGGCACGGAAACGGGCGGCAATGCGTCGAGCACGGCCGTGCGTGCGCTGATCAAGCAGTTC
GTCGCGTCGGAGAGCCCGGCCAAGCCGCTGTCGGACAGCCAGATCGCGGAGATGCTCAAGGAGCAGGGCATCGAATGCGC
GCGCCGCACGGTGGCGAAATACCGCGAGGCGCTGAAGATCGCACCGGCCAACCTGCGCAAGGCGCTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-356)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1TJ88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.731

100

0.529