Detailed information NTG0621983

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LJC78_RS13260
• Genome accession: NZ_CP086007
• Coordinates: 2458194..2458811 (-)
• Length: 205 a.a.
• NCBI ID: WP_014480312.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. natto strain SCP010-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2453194..2463811

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LJC78_RS13230 (LJC78_13235)	rpsT	2453496..2453762 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
LJC78_RS13235 (LJC78_13240)	holA	2453777..2454820 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
LJC78_RS13240 (LJC78_13245)	-	2454860..2455009 (-)	150	WP_003229985.1	hypothetical protein	-
LJC78_RS13245 (LJC78_13250)	yqzM	2455050..2455184 (+)	135	WP_003229983.1	YqzM family protein	-
LJC78_RS13250 (LJC78_13255)	comEC	2455224..2457554 (-)	2331	WP_033881047.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LJC78_RS13255 (LJC78_13260)	comEB	2457558..2458127 (-)	570	WP_003229978.1	ComE operon protein 2	-
LJC78_RS13260 (LJC78_13265)	comEA	2458194..2458811 (-)	618	WP_014480312.1	competence protein ComEA	Machinery gene
LJC78_RS13265 (LJC78_13270)	comER	2458895..2459716 (+)	822	WP_014480313.1	late competence protein ComER	-
LJC78_RS13270 (LJC78_13275)	yqeM	2459782..2460525 (-)	744	WP_014480314.1	class I SAM-dependent DNA methyltransferase	-
LJC78_RS13275 (LJC78_13280)	rsfS	2460522..2460878 (-)	357	WP_014480315.1	ribosome silencing factor	-
LJC78_RS13280 (LJC78_13285)	yqeK	2460896..2461456 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LJC78_RS13285 (LJC78_13290)	nadD	2461446..2462015 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
LJC78_RS13290 (LJC78_13295)	yhbY	2462027..2462317 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
LJC78_RS13295 (LJC78_13300)	aroE	2462311..2463153 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21781.51 Da
• Isoelectric Point: 4.7220

>NTDB_id=621983 LJC78_RS13260 WP_014480312.1 2458194..2458811(-) (comEA) [Bacillus subtilis subsp. natto strain SCP010-1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=621983 LJC78_RS13260 WP_014480312.1 2458194..2458811(-) (comEA) [Bacillus subtilis subsp. natto strain SCP010-1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	99.512	100	0.995
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39