Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIT35_RS16665 Genome accession   NZ_CP085720
Coordinates   3376265..3377308 (-) Length   347 a.a.
NCBI ID   WP_127761365.1    Uniprot ID   A0A3Q9RPP5
Organism   Peribacillus asahii strain KF4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3371265..3382308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT35_RS16625 (LIT35_16705) - 3372072..3372866 (+) 795 WP_127761358.1 YqhG family protein -
  LIT35_RS16630 (LIT35_16710) - 3372946..3373143 (-) 198 WP_119115514.1 YqzE family protein -
  LIT35_RS16635 (LIT35_16715) comGG 3373373..3373753 (-) 381 WP_127761359.1 competence type IV pilus minor pilin ComGG -
  LIT35_RS16640 (LIT35_16720) comGF 3373743..3374189 (-) 447 WP_127761360.1 competence type IV pilus minor pilin ComGF -
  LIT35_RS16645 (LIT35_16725) - 3374173..3374511 (-) 339 WP_127761361.1 hypothetical protein -
  LIT35_RS16650 (LIT35_16730) comGD 3374498..3374932 (-) 435 WP_127761362.1 competence type IV pilus minor pilin ComGD -
  LIT35_RS16655 (LIT35_16735) comGC 3374919..3375257 (-) 339 WP_127761363.1 competence type IV pilus major pilin ComGC -
  LIT35_RS16660 (LIT35_16740) comGB 3375247..3376299 (-) 1053 WP_127761364.1 competence type IV pilus assembly protein ComGB -
  LIT35_RS16665 (LIT35_16745) comGA 3376265..3377308 (-) 1044 WP_127761365.1 competence type IV pilus ATPase ComGA Machinery gene
  LIT35_RS16670 (LIT35_16750) - 3377759..3378460 (+) 702 WP_127761366.1 helix-turn-helix domain-containing protein -
  LIT35_RS16675 (LIT35_16755) - 3378547..3378789 (+) 243 WP_127761367.1 DUF2626 domain-containing protein -
  LIT35_RS16680 (LIT35_16760) - 3378835..3379911 (-) 1077 WP_127761368.1 SAM-dependent methyltransferase -
  LIT35_RS16685 (LIT35_16765) - 3379992..3381161 (-) 1170 WP_252282574.1 M20 family metallopeptidase -
  LIT35_RS16690 (LIT35_16770) - 3381207..3381830 (-) 624 WP_127761370.1 MBL fold metallo-hydrolase -
  LIT35_RS16695 (LIT35_16775) - 3382042..3382215 (+) 174 WP_119115525.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38989.05 Da        Isoelectric Point: 9.1414

>NTDB_id=619627 LIT35_RS16665 WP_127761365.1 3376265..3377308(-) (comGA) [Peribacillus asahii strain KF4]
MSIEKTADDILKRAIRLAASDIHILPRKEGPLVRFRIDNRLIPQENLSFEETERLISHLKYLASMDIGEKRRPQNGSITL
HLEDRVIGLRLSTLPTAYVESLAIRLIPQQNILPLNQLSLFPTTTERLISLLKHSHGMIIFTGPTGSGKTTTLYSILHHV
QELYNRHVITLEDPIENQSENVLQVQINEKAGITYSVGLKAALRHDPDVIMVGEIRDSETAKIAIRAALTGHLLLTTMHS
RDAAGAISRLLEFGVSWLEIEQSLIAVTAQRLVELRNRRTVGGAANKRASVYELLYGRDLVKVLQGVKGENIEARHRTLK
EEIGKAVAMGFVENKEFERLVFIDERG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=619627 LIT35_RS16665 WP_127761365.1 3376265..3377308(-) (comGA) [Peribacillus asahii strain KF4]
ATGTCTATTGAAAAAACGGCAGATGATATTTTGAAGCGTGCTATCAGGTTAGCTGCATCAGATATTCATATTTTGCCTCG
CAAAGAAGGTCCACTCGTTCGATTTCGAATCGATAATCGACTCATTCCCCAAGAAAATCTCTCTTTTGAAGAAACAGAAC
GTCTTATCTCTCATCTAAAATATCTCGCATCAATGGATATCGGTGAAAAAAGAAGACCCCAAAACGGTTCGATTACGCTT
CATTTAGAAGATCGAGTGATCGGACTTAGATTATCAACATTACCTACAGCATATGTTGAAAGTTTAGCCATTCGTTTAAT
TCCCCAGCAAAACATTCTGCCACTTAACCAACTCTCTCTTTTTCCAACCACTACCGAGCGATTGATTTCTCTTTTAAAGC
ACTCACATGGAATGATTATTTTCACAGGTCCGACTGGATCAGGTAAAACGACAACGCTGTACTCCATCCTTCATCATGTG
CAGGAATTGTATAACCGCCATGTTATTACTCTTGAAGATCCTATCGAAAATCAATCCGAAAACGTATTGCAAGTTCAAAT
TAATGAGAAAGCGGGAATTACGTATTCAGTCGGCTTAAAGGCAGCTTTACGTCACGATCCTGATGTGATTATGGTGGGAG
AAATTCGTGATTCAGAAACAGCTAAAATAGCGATTAGGGCGGCACTTACAGGCCATTTACTGTTAACAACGATGCATAGC
CGCGATGCCGCAGGAGCTATTTCACGGCTGTTAGAGTTTGGTGTAAGTTGGTTAGAAATTGAACAAAGTTTAATTGCTGT
AACGGCACAACGTTTAGTCGAATTAAGAAATCGAAGAACAGTAGGCGGTGCTGCAAATAAGCGAGCAAGTGTGTATGAAC
TTCTTTATGGAAGAGATTTAGTGAAAGTGTTACAAGGAGTAAAAGGAGAGAATATTGAAGCTCGCCATCGAACGTTGAAA
GAAGAAATCGGTAAAGCTGTAGCGATGGGCTTTGTCGAGAACAAAGAATTTGAAAGATTGGTATTTATTGATGAGAGGGG
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9RPP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

53.429

100

0.539
  pilB Haemophilus influenzae 86-028NP

39.053

97.406

0.38
  pilB Haemophilus influenzae Rd KW20

38.166

97.406

0.372
  pilB Glaesserella parasuis strain SC1401

42.333

86.455

0.366