Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIS76_RS16700 Genome accession   NZ_CP085717
Coordinates   3420171..3421214 (-) Length   347 a.a.
NCBI ID   WP_252290779.1    Uniprot ID   -
Organism   Peribacillus asahii strain CMF4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3415171..3426214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS76_RS16660 (LIS76_16715) - 3415254..3415424 (-) 171 WP_252290765.1 hypothetical protein -
  LIS76_RS16665 (LIS76_16720) - 3415493..3416401 (-) 909 WP_252290767.1 hypothetical protein -
  LIS76_RS16670 (LIS76_16725) - 3416541..3416723 (-) 183 WP_252290769.1 hypothetical protein -
  LIS76_RS16675 (LIS76_16730) - 3416867..3417115 (-) 249 WP_252290771.1 hypothetical protein -
  LIS76_RS16680 (LIS76_16735) - 3417502..3417951 (-) 450 WP_252290773.1 hypothetical protein -
  LIS76_RS16685 (LIS76_16740) - 3418099..3418710 (-) 612 WP_252290775.1 helix-turn-helix transcriptional regulator -
  LIS76_RS16690 (LIS76_16745) comGC 3418984..3419163 (-) 180 Protein_3242 competence type IV pilus major pilin ComGC -
  LIS76_RS16695 (LIS76_16750) comGB 3419153..3420205 (-) 1053 WP_252290777.1 competence type IV pilus assembly protein ComGB -
  LIS76_RS16700 (LIS76_16755) comGA 3420171..3421214 (-) 1044 WP_252290779.1 competence type IV pilus ATPase ComGA Machinery gene
  LIS76_RS16705 (LIS76_16760) - 3421667..3422368 (+) 702 WP_252290781.1 helix-turn-helix domain-containing protein -
  LIS76_RS16710 (LIS76_16765) - 3422456..3422698 (+) 243 WP_119115521.1 DUF2626 domain-containing protein -
  LIS76_RS16715 (LIS76_16770) - 3422744..3423820 (-) 1077 WP_252290783.1 SAM-dependent methyltransferase -
  LIS76_RS16720 (LIS76_16775) - 3423900..3425069 (-) 1170 WP_252290785.1 M20 family metallopeptidase -
  LIS76_RS16725 (LIS76_16780) - 3425115..3425738 (-) 624 WP_252290787.1 MBL fold metallo-hydrolase -
  LIS76_RS16730 (LIS76_16785) - 3425950..3426123 (+) 174 WP_119115525.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38932.04 Da        Isoelectric Point: 9.1325

>NTDB_id=619581 LIS76_RS16700 WP_252290779.1 3420171..3421214(-) (comGA) [Peribacillus asahii strain CMF4]
MSIEKTADDILKRAIRLAASDIHILPRKEGPLVRFRIDNRLIPQETLSFEETERLISHLKYLASMDIGEKRRPQNGSITL
HLEDRVIGLRLSTLPTAYVESLAIRLIPQQNILPLNQLSLFPTTTERLISLLKHSHGMIIFTGPTGSGKTTTLYSILHHV
QELYNRHVITLEDPIENQSENVLQVQINEKAGITYSVGLKAALRHDPDVIMVGEIRDSETAKIAIRAALTGHLLLTTMHS
RDAAGAISRLLEFGVSWLEIEQSLIAVTAQRLVELKNRRIAGGAANKRASVYELLYGRDLVKVLQGVKGENIEARHRTLK
EEIGKAVAMGFVENKEFERLVFIDERG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=619581 LIS76_RS16700 WP_252290779.1 3420171..3421214(-) (comGA) [Peribacillus asahii strain CMF4]
ATGTCTATTGAAAAAACGGCAGATGATATTTTGAAGCGTGCTATCAGGTTAGCTGCATCAGATATTCATATTTTGCCTCG
CAAAGAAGGTCCGCTCGTTCGATTTCGAATCGATAATCGACTCATTCCCCAAGAAACTCTCTCTTTTGAAGAAACAGAAC
GTCTTATCTCTCATCTAAAATATCTCGCATCAATGGATATCGGTGAAAAAAGAAGACCCCAAAACGGTTCGATTACGCTT
CATTTAGAAGATCGAGTGATTGGACTTAGATTATCAACATTACCTACAGCATATGTTGAAAGTTTAGCCATTCGTTTAAT
TCCCCAGCAAAACATTCTGCCACTTAACCAACTCTCTCTTTTTCCAACCACTACCGAGCGATTGATTTCTCTTTTAAAGC
ACTCACATGGAATGATTATTTTCACAGGTCCGACTGGATCAGGTAAAACGACAACGCTGTACTCCATCCTTCATCATGTG
CAGGAATTGTATAACCGCCATGTTATTACTCTTGAAGATCCTATCGAAAATCAATCCGAAAACGTATTGCAAGTTCAAAT
TAATGAGAAAGCAGGAATTACGTATTCAGTTGGTTTAAAGGCAGCTTTACGTCACGATCCTGATGTGATTATGGTGGGAG
AAATTCGTGATTCAGAAACAGCTAAAATAGCCATTAGGGCGGCACTTACAGGCCATTTACTGTTAACAACGATGCATAGC
CGCGATGCCGCAGGAGCTATTTCACGGCTGTTAGAGTTTGGTGTAAGTTGGTTAGAAATTGAACAAAGTTTAATTGCTGT
AACGGCACAGCGTTTAGTCGAATTAAAAAATCGAAGAATAGCAGGCGGTGCTGCAAATAAGCGAGCAAGTGTGTATGAAC
TTCTTTATGGAAGAGATTTAGTGAAAGTGTTACAAGGAGTAAAAGGAGAGAATATTGAAGCTCGCCATCGAACGTTGAAA
GAAGAAATCGGTAAAGCTGTAGCGATGGGCTTTGTTGAGAACAAAGAATTTGAAAGATTGGTATTTATTGATGAGAGGGG
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

53.429

100

0.539
  pilB Haemophilus influenzae 86-028NP

39.053

97.406

0.38
  pilB Haemophilus influenzae Rd KW20

38.166

97.406

0.372
  pilB Glaesserella parasuis strain SC1401

42.333

86.455

0.366