Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LIT36_RS12350 Genome accession   NZ_CP085706
Coordinates   2538638..2539252 (-) Length   204 a.a.
NCBI ID   WP_286674421.1    Uniprot ID   -
Organism   Bacillus velezensis strain CK17     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2533638..2544252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT36_RS12320 (LIT36_12330) rpsT 2533938..2534204 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  LIT36_RS12325 (LIT36_12335) holA 2534221..2535262 (-) 1042 Protein_2384 DNA polymerase III subunit delta -
  LIT36_RS12330 (LIT36_12340) - 2535302..2535456 (-) 155 Protein_2385 hypothetical protein -
  LIT36_RS12335 (LIT36_12345) - 2535497..2535631 (+) 135 WP_003152870.1 YqzM family protein -
  LIT36_RS12340 (LIT36_12350) comEC 2535650..2538001 (-) 2352 WP_201489015.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LIT36_RS12345 (LIT36_12355) - 2538002..2538571 (-) 570 WP_003152868.1 ComE operon protein 2 -
  LIT36_RS12350 (LIT36_12360) comEA 2538638..2539252 (-) 615 WP_286674421.1 helix-hairpin-helix domain-containing protein Machinery gene
  LIT36_RS12355 (LIT36_12365) comER 2539311..2540132 (+) 822 WP_044053476.1 late competence protein ComER -
  LIT36_RS12360 (LIT36_12370) - 2540201..2540938 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  LIT36_RS12365 (LIT36_12375) rsfS 2540935..2541291 (-) 357 WP_003152864.1 ribosome silencing factor -
  LIT36_RS12370 (LIT36_12380) yqeK 2541288..2541869 (-) 582 WP_003152863.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  LIT36_RS12375 (LIT36_12385) - 2541859..2542428 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  LIT36_RS12380 (LIT36_12390) yhbY 2542439..2542729 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  LIT36_RS12385 (LIT36_12395) aroE 2542723..2543565 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22192.40 Da        Isoelectric Point: 6.9685

>NTDB_id=619436 LIT36_RS12350 WP_286674421.1 2538638..2539252(-) (comEA) [Bacillus velezensis strain CK17]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKISGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=619436 LIT36_RS12350 WP_286674421.1 2538638..2539252(-) (comEA) [Bacillus velezensis strain CK17]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGATATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363