Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SIR_RS16200 Genome accession   NC_022246
Coordinates   1324231..1325532 (-) Length   433 a.a.
NCBI ID   WP_020998970.1    Uniprot ID   T1ZEG0
Organism   Streptococcus intermedius B196     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1319231..1330532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIR_RS16170 (SIR_1263) - 1319857..1320420 (-) 564 WP_004234076.1 YutD family protein -
  SIR_RS16175 (SIR_1264) sepM 1320687..1321742 (-) 1056 WP_003073620.1 SepM family pheromone-processing serine protease Regulator
  SIR_RS16180 (SIR_1265) coaD 1321723..1322220 (-) 498 WP_003078294.1 pantetheine-phosphate adenylyltransferase -
  SIR_RS16185 (SIR_1266) rsmD 1322210..1322749 (-) 540 WP_021003032.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SIR_RS16190 (SIR_1267) hpf 1322948..1323490 (-) 543 WP_003073626.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SIR_RS16195 (SIR_1268) - 1323569..1324234 (-) 666 WP_021003033.1 ComF family protein -
  SIR_RS16200 (SIR_1269) comFA/cflA 1324231..1325532 (-) 1302 WP_020998970.1 DEAD/DEAH box helicase Machinery gene
  SIR_RS16205 (SIR_1270) - 1325589..1326224 (+) 636 WP_020998971.1 YigZ family protein -
  SIR_RS16210 (SIR_1271) cysK 1326323..1327252 (+) 930 WP_020998972.1 cysteine synthase A -
  SIR_RS20225 - 1327457..1327732 (-) 276 Protein_1263 cell filamentation protein Fic -
  SIR_RS16220 (SIR_1272) - 1327944..1328090 (-) 147 WP_020998973.1 hypothetical protein -
  SIR_RS20045 (SIR_1273) - 1328172..1328333 (-) 162 WP_003070190.1 DUF6290 family protein -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49245.04 Da        Isoelectric Point: 8.9799

>NTDB_id=61891 SIR_RS16200 WP_020998970.1 1324231..1325532(-) (comFA/cflA) [Streptococcus intermedius B196]
MIELQDCLGRIFTRSQLPLGLQQQAQTLSGMIEKKGKLWCNRCGQVVHKEKQQLPIGAYYCRPCVRLGRVRSDEKLYYFP
QAEFPKTNVLKWEGRLTDYQAKVSQGLVEAVAKQKNSLVHAVTGAGKTEMIYQVVAQVINEGGAVCLASPRIDVCLELHR
RLQEDFSCDISLLHGESEVYFRSPLVIATTHQLLKFYRAFDLLIVDEVDAFPYVDNPMLYHAVAQSVKEKSTIIFLTATS
TDELDKKVSKGELNRLSLPRRFHGNPLIVPQKVWLENFQKYLNQKKLVPKLEQFVKKQRKTGFPLLIFASEIKRGQELAE
VLQSYFSSENVGFVASTTENRLDIVEKFRQKEITILVTTTILERGVTFPCVDVFVVEANHRLFSRSALVQIAGRVGRSMD
RPTGELLFFHDGTNFAIERAIQEIKAMNQEAGL

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=61891 SIR_RS16200 WP_020998970.1 1324231..1325532(-) (comFA/cflA) [Streptococcus intermedius B196]
ATGATAGAATTACAAGACTGTTTAGGTCGTATCTTTACAAGAAGCCAATTACCATTAGGATTGCAGCAACAAGCTCAAAC
ATTGTCGGGAATGATAGAAAAAAAGGGGAAATTATGGTGCAATCGTTGTGGACAAGTAGTTCATAAAGAAAAACAGCAAC
TACCAATAGGTGCTTATTATTGCAGGCCATGCGTGCGATTAGGAAGGGTCAGAAGTGATGAAAAACTCTACTATTTTCCA
CAGGCAGAGTTCCCAAAAACGAATGTTTTGAAATGGGAAGGAAGGTTAACAGATTACCAAGCTAAGGTTTCTCAAGGTCT
TGTAGAGGCAGTTGCCAAACAAAAAAATAGCCTGGTTCATGCTGTCACAGGAGCAGGAAAAACAGAAATGATCTATCAAG
TGGTGGCACAAGTTATCAATGAAGGTGGTGCAGTTTGCTTGGCTAGCCCTCGAATTGATGTCTGTCTAGAACTACATCGC
AGATTGCAAGAAGATTTTAGTTGTGATATTTCACTCCTGCACGGTGAATCAGAAGTTTATTTTCGCAGTCCTCTTGTGAT
TGCCACAACGCATCAGCTTCTCAAATTTTACCGAGCATTTGATCTTCTTATTGTTGACGAGGTGGATGCCTTTCCTTATG
TGGATAATCCGATGCTTTACCATGCCGTTGCACAATCAGTAAAAGAGAAAAGTACTATAATTTTTCTAACTGCAACCTCT
ACAGATGAGCTGGATAAAAAAGTTAGCAAGGGAGAGTTGAACCGCTTAAGTCTCCCTAGACGTTTTCATGGTAACCCTTT
AATTGTTCCTCAAAAAGTTTGGTTGGAAAATTTTCAAAAATACTTGAATCAAAAAAAGCTAGTTCCTAAGTTGGAACAAT
TTGTTAAAAAGCAAAGAAAAACAGGTTTTCCTCTTCTCATTTTTGCTTCTGAGATTAAAAGAGGACAAGAATTGGCAGAG
GTTCTCCAAAGTTATTTTTCCAGTGAGAACGTTGGCTTTGTAGCCTCAACGACTGAAAATCGACTCGATATTGTAGAGAA
ATTTCGACAAAAAGAAATTACAATTTTAGTAACAACGACAATTTTAGAACGTGGTGTGACTTTTCCTTGTGTAGATGTTT
TTGTGGTGGAGGCTAACCACCGTTTGTTTAGTCGCAGTGCTCTGGTACAAATAGCTGGACGCGTTGGTCGCAGTATGGAT
CGTCCGACAGGTGAGCTACTCTTTTTTCATGATGGGACCAATTTTGCTATTGAACGAGCTATTCAGGAAATTAAGGCGAT
GAATCAGGAGGCTGGTTTATGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(98-245)

Helicase conserved C-terminal domain (Helicase_C)

(292-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB T1ZEG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

69.626

98.845

0.688
  comFA/cflA Streptococcus pneumoniae D39

69.626

98.845

0.688
  comFA/cflA Streptococcus pneumoniae R6

69.626

98.845

0.688
  comFA/cflA Streptococcus pneumoniae TIGR4

69.393

98.845

0.686
  comFA/cflA Streptococcus mitis NCTC 12261

69.718

98.383

0.686
  comFA/cflA Streptococcus mitis SK321

69.249

98.383

0.681
  comFA Lactococcus lactis subsp. cremoris KW2

54.02

91.917

0.497
  comFA Latilactobacillus sakei subsp. sakei 23K

40.233

99.307

0.4

Multiple sequence alignment