Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIT31_RS16270 Genome accession   NZ_CP085514
Coordinates   3427140..3428216 (-) Length   358 a.a.
NCBI ID   WP_252281222.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans strain KF19     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3422140..3433216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT31_RS16230 (LIT31_16295) - 3423007..3423804 (+) 798 WP_252277960.1 YqhG family protein -
  LIT31_RS16235 (LIT31_16300) - 3423850..3424035 (-) 186 WP_057278811.1 YqzE family protein -
  LIT31_RS16240 (LIT31_16305) comGG 3424152..3424538 (-) 387 WP_252277961.1 competence type IV pilus minor pilin ComGG -
  LIT31_RS16245 (LIT31_16310) comGF 3424531..3425010 (-) 480 WP_252277962.1 competence type IV pilus minor pilin ComGF -
  LIT31_RS16250 (LIT31_16315) - 3424958..3425296 (-) 339 WP_214773268.1 hypothetical protein -
  LIT31_RS16255 (LIT31_16320) comGD 3425283..3425741 (-) 459 WP_252277963.1 competence type IV pilus minor pilin ComGD -
  LIT31_RS16260 (LIT31_16325) comGC 3425734..3426045 (-) 312 WP_286676168.1 competence type IV pilus major pilin ComGC Machinery gene
  LIT31_RS16265 (LIT31_16330) comGB 3426122..3427156 (-) 1035 WP_252277964.1 competence type IV pilus assembly protein ComGB -
  LIT31_RS16270 (LIT31_16335) comGA 3427140..3428216 (-) 1077 WP_252281222.1 competence type IV pilus ATPase ComGA Machinery gene
  LIT31_RS16275 (LIT31_16340) - 3428320..3428700 (-) 381 WP_252277965.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LIT31_RS16280 (LIT31_16345) - 3428916..3429617 (+) 702 WP_252277966.1 helix-turn-helix domain-containing protein -
  LIT31_RS16285 (LIT31_16350) - 3429703..3429945 (+) 243 WP_034308393.1 DUF2626 domain-containing protein -
  LIT31_RS16290 (LIT31_16355) - 3429998..3431098 (-) 1101 WP_252277967.1 SAM-dependent methyltransferase -
  LIT31_RS16295 (LIT31_16360) - 3431353..3431979 (-) 627 WP_057278817.1 MBL fold metallo-hydrolase -
  LIT31_RS16300 (LIT31_16365) - 3432213..3432386 (+) 174 WP_034308396.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39800.40 Da        Isoelectric Point: 8.4751

>NTDB_id=618775 LIT31_RS16270 WP_252281222.1 3427140..3428216(-) (comGA) [Peribacillus frigoritolerans strain KF19]
MISIEKTAEKILTRAIVESASDIHIFFRGGGPHIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQTLIGVTAQRLVELRCLPCKGDCALACKMTERNKRASVYELLYGKSLAEVLRIMGDEK
GKASVSYRQLKDEIGKAVAMGYVESQEYERLVYHETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=618775 LIT31_RS16270 WP_252281222.1 3427140..3428216(-) (comGA) [Peribacillus frigoritolerans strain KF19]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTAACCCGTGCCATCGTGGAATCGGCATCGGATATCCACATTTTTTT
TCGCGGAGGGGGGCCTCACATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACATTATCATTCTTTGAAGCTG
AAAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGTGCCATCACC
ATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTGCACTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACTTTATATTCCCTGCTTCACCAC
GCTCATGAGATGATCAATCGAAATATCATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTATTGCAAGTCCA
AATCAATGAAAAGGCGGGCATTACGTATTCAGTCGGCCTAAAAGCTGTTCTGAGGCATGACCCGGATGTGATCATGGTTG
GGGAAGTCAGGGATGCAGAAACTGCCAAAATCGCAGTCCGTGCCGCATTAACCGGTCATTTGATACTAACGACCATGCAT
ACTAGGGATGCCCAGGGCGCCATCTCCAGATTACTGGAATTTGGTGTCAGCTTACTTGAGGTTGAACAGACTTTGATTGG
CGTGACAGCTCAGCGGCTGGTTGAATTGCGATGTCTTCCGTGTAAAGGGGACTGTGCTTTAGCTTGCAAAATGACTGAAA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGGAAAAGTCTGGCTGAAGTTCTCCGGATAATGGGAGATGAAAAA
GGAAAGGCATCGGTCAGCTACCGCCAATTAAAGGATGAAATCGGAAAAGCGGTTGCGATGGGCTATGTGGAATCTCAGGA
ATATGAACGGCTGGTATACCATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

37.644

97.207

0.366
  pilB Haemophilus influenzae Rd KW20

37.069

97.207

0.36