Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LIT34_RS20100 Genome accession   NZ_CP085431
Coordinates   3863223..3863597 (+) Length   124 a.a.
NCBI ID   WP_223269005.1    Uniprot ID   -
Organism   Priestia megaterium strain F20     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3858223..3868597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT34_RS20080 (LIT34_20110) - 3858714..3859385 (-) 672 WP_013058558.1 response regulator transcription factor -
  LIT34_RS20085 (LIT34_20115) - 3859711..3860529 (+) 819 WP_013058559.1 phytoene/squalene synthase family protein -
  LIT34_RS20090 (LIT34_20120) - 3860565..3862514 (-) 1950 WP_041816612.1 FUSC family protein -
  LIT34_RS20095 (LIT34_20125) - 3862670..3862930 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  LIT34_RS20100 (LIT34_20130) nucA/comI 3863223..3863597 (+) 375 WP_223269005.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LIT34_RS20105 (LIT34_20135) comJ 3863633..3864040 (+) 408 WP_013058563.1 competence protein ComJ -
  LIT34_RS20110 (LIT34_20140) helD 3864069..3866387 (-) 2319 WP_013058564.1 RNA polymerase recycling motor HelD -
  LIT34_RS32215 - 3866632..3866757 (-) 126 WP_016765178.1 hypothetical protein -
  LIT34_RS20115 (LIT34_20145) - 3866897..3867415 (-) 519 WP_235565579.1 enoyl-CoA hydratase/isomerase family protein -
  LIT34_RS20120 (LIT34_20150) - 3867655..3868023 (+) 369 WP_029323759.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13661.90 Da        Isoelectric Point: 4.2601

>NTDB_id=618090 LIT34_RS20100 WP_223269005.1 3863223..3863597(+) (nucA/comI) [Priestia megaterium strain F20]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=618090 LIT34_RS20100 WP_223269005.1 3863223..3863597(+) (nucA/comI) [Priestia megaterium strain F20]
ATGGGACATGCAGCTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAACTATGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGGGAAGAATCGCTGAAAGGATATCCGACAAAAACAGGCTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645