Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIT26_RS18820 Genome accession   NZ_CP085395
Coordinates   3910454..3911530 (-) Length   358 a.a.
NCBI ID   WP_063595034.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans strain CK6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3905454..3916530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT26_RS18780 (LIT26_18795) - 3906344..3907141 (+) 798 WP_048688306.1 YqhG family protein -
  LIT26_RS18785 (LIT26_18800) - 3907188..3907373 (-) 186 WP_048688303.1 YqzE family protein -
  LIT26_RS18790 (LIT26_18805) comGG 3907466..3907852 (-) 387 WP_048688300.1 competence type IV pilus minor pilin ComGG -
  LIT26_RS18795 (LIT26_18810) comGF 3907845..3908324 (-) 480 WP_081088666.1 competence type IV pilus minor pilin ComGF -
  LIT26_RS18800 (LIT26_18815) - 3908272..3908610 (-) 339 WP_252267050.1 hypothetical protein -
  LIT26_RS18805 (LIT26_18820) comGD 3908597..3909040 (-) 444 WP_063594105.1 competence type IV pilus minor pilin ComGD -
  LIT26_RS18810 (LIT26_18825) comGC 3909048..3909359 (-) 312 WP_283778890.1 competence type IV pilus major pilin ComGC -
  LIT26_RS18815 (LIT26_18830) comGB 3909436..3910470 (-) 1035 WP_063595035.1 competence type IV pilus assembly protein ComGB -
  LIT26_RS18820 (LIT26_18835) comGA 3910454..3911530 (-) 1077 WP_063595034.1 competence type IV pilus ATPase ComGA Machinery gene
  LIT26_RS18825 (LIT26_18840) - 3911635..3912015 (-) 381 WP_063594153.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LIT26_RS18830 (LIT26_18845) - 3912231..3912932 (+) 702 WP_063594154.1 helix-turn-helix domain-containing protein -
  LIT26_RS18835 (LIT26_18850) - 3913019..3913261 (+) 243 WP_063594155.1 DUF2626 domain-containing protein -
  LIT26_RS18840 (LIT26_18855) - 3913314..3914414 (-) 1101 WP_063595036.1 SAM-dependent methyltransferase -
  LIT26_RS18845 (LIT26_18860) - 3914728..3915354 (-) 627 WP_063594156.1 MBL fold metallo-hydrolase -
  LIT26_RS18850 (LIT26_18865) - 3915589..3915762 (+) 174 WP_065310842.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39791.39 Da        Isoelectric Point: 7.8587

>NTDB_id=617562 LIT26_RS18820 WP_063595034.1 3910454..3911530(-) (comGA) [Peribacillus frigoritolerans strain CK6]
MISIEKTAEKILTRAIVESASDIHIFFRGEGPLIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQTLIGVTAQRLVELRCLPCKGDCALACKMTARNKRASVYELLYGKSLAEVLRIMGDEK
GEATVSYRQLKDEIGKAVAMGYVDSQEYERLVYHETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=617562 LIT26_RS18820 WP_063595034.1 3910454..3911530(-) (comGA) [Peribacillus frigoritolerans strain CK6]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTAACCCGTGCCATCGTGGAGTCGGCATCGGATATCCACATTTTTTT
TCGCGGAGAGGGGCCTCTCATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACCTTATCATTCTTTGAAGCTG
AAAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGCGCCATCACT
ATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTGCACTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACATTATATTCCCTGCTTCACCAC
GCCCATGAGATGATCAATCGAAATATCATTACACTTGAAGACCCCATTGAAAATGTATCCGAAAAGGTATTGCAAGTCCA
AATCAATGAAAAGGCGGGCATTACGTATTCCGTCGGCCTAAAAGCTGTTCTGAGGCATGACCCGGACGTGATCATGGTTG
GGGAAGTCAGGGATGCAGAAACTGCCAAAATCGCAGTCCGTGCCGCATTAACCGGTCATTTGATACTAACGACCATGCAT
ACGAGGGACGCCCAGGGTGCCATCTCCAGATTACTGGAATTTGGTGTCAGCTTACTTGAGGTTGAACAGACTTTGATTGG
CGTGACAGCGCAGCGGCTTGTTGAATTGCGATGTCTTCCGTGTAAAGGAGACTGTGCTTTAGCTTGCAAAATGACTGCGA
GGAATAAAAGGGCAAGTGTATATGAATTGCTCTATGGAAAAAGTCTGGCTGAAGTTCTCCGGATAATGGGAGATGAAAAA
GGAGAGGCAACGGTCAGCTACCGCCAATTAAAGGATGAAATCGGAAAAGCGGTTGCGATGGGCTATGTGGATTCTCAGGA
ATATGAACGGCTGGTATACCATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

38.506

97.207

0.374
  pilB Haemophilus influenzae Rd KW20

37.356

97.207

0.363