NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	LIS75_RS12345	Genome accession	NZ_CP085388
Coordinates	2538475..2539089 (-)	Length	204 a.a.
NCBI ID	WP_286674421.1	Uniprot ID	-
Organism	Bacillus velezensis strain CMF18
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2533475..2544089

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LIS75_RS12315 (LIS75_12325)	rpsT	2533775..2534041 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LIS75_RS12320 (LIS75_12330)	holA	2534058..2535099 (-)	1042	Protein_2383	DNA polymerase III subunit delta	-
LIS75_RS12325 (LIS75_12335)	-	2535139..2535293 (-)	155	Protein_2384	hypothetical protein	-
LIS75_RS12330 (LIS75_12340)	-	2535334..2535468 (+)	135	WP_003152870.1	YqzM family protein	-
LIS75_RS12335 (LIS75_12345)	comEC	2535487..2537838 (-)	2352	WP_201489015.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LIS75_RS12340 (LIS75_12350)	-	2537839..2538408 (-)	570	WP_003152868.1	ComE operon protein 2	-
LIS75_RS12345 (LIS75_12355)	comEA	2538475..2539089 (-)	615	WP_286674421.1	helix-hairpin-helix domain-containing protein	Machinery gene
LIS75_RS12350 (LIS75_12360)	comER	2539148..2539969 (+)	822	WP_044053476.1	late competence protein ComER	-
LIS75_RS12355 (LIS75_12365)	-	2540038..2540775 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
LIS75_RS12360 (LIS75_12370)	rsfS	2540772..2541128 (-)	357	WP_003152864.1	ribosome silencing factor	-
LIS75_RS12365 (LIS75_12375)	yqeK	2541125..2541706 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LIS75_RS12370 (LIS75_12380)	-	2541696..2542265 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
LIS75_RS12375 (LIS75_12385)	yhbY	2542276..2542566 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
LIS75_RS12380 (LIS75_12390)	aroE	2542560..2543402 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22192.40 Da Isoelectric Point: 6.9685

                                >NTDB_id=617292 LIS75_RS12345 WP_286674421.1 2538475..2539089(-) (comEA) [Bacillus velezensis strain CMF18]

MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMKISGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=617292 LIS75_RS12345 WP_286674421.1 2538475..2539089(-) (comEA) [Bacillus velezensis strain CMF18]

ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG

CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGATATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0617292