Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LJA38_RS12280 Genome accession   NZ_CP085283
Coordinates   2562822..2563436 (-) Length   204 a.a.
NCBI ID   WP_015387983.1    Uniprot ID   A0A6A8LHQ3
Organism   Bacillus velezensis strain TPS3N     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2557822..2568436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJA38_RS12250 rpsT 2558122..2558388 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  LJA38_RS12255 holA 2558405..2559446 (-) 1042 Protein_2372 DNA polymerase III subunit delta -
  LJA38_RS12260 - 2559486..2559640 (-) 155 Protein_2373 hypothetical protein -
  LJA38_RS12265 - 2559681..2559815 (+) 135 WP_003152870.1 YqzM family protein -
  LJA38_RS12270 comEC 2559834..2562185 (-) 2352 WP_063174744.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LJA38_RS12275 - 2562186..2562755 (-) 570 WP_003152868.1 ComE operon protein 2 -
  LJA38_RS12280 comEA 2562822..2563436 (-) 615 WP_015387983.1 helix-hairpin-helix domain-containing protein Machinery gene
  LJA38_RS12285 comER 2563495..2564316 (+) 822 WP_014305449.1 late competence protein ComER -
  LJA38_RS12290 - 2564385..2565122 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  LJA38_RS12295 rsfS 2565119..2565475 (-) 357 WP_003152864.1 ribosome silencing factor -
  LJA38_RS12300 yqeK 2565472..2566053 (-) 582 WP_063174743.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  LJA38_RS12305 - 2566043..2566612 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  LJA38_RS12310 yhbY 2566623..2566913 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  LJA38_RS12315 aroE 2566907..2567749 (-) 843 WP_025852995.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22255.41 Da        Isoelectric Point: 5.5211

>NTDB_id=616985 LJA38_RS12280 WP_015387983.1 2562822..2563436(-) (comEA) [Bacillus velezensis strain TPS3N]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=616985 LJA38_RS12280 WP_015387983.1 2562822..2563436(-) (comEA) [Bacillus velezensis strain TPS3N]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGTGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCTATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363