Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   NR351_RS15550 Genome accession   NZ_CP102378
Coordinates   3180464..3181123 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain JB41     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3175464..3186123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NR351_RS15525 (NR351_15525) ygiS 3176747..3178354 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  NR351_RS15530 (NR351_15530) mqsA 3178487..3178882 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  NR351_RS15535 (NR351_15535) mqsR 3178884..3179180 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  NR351_RS15540 (NR351_15540) ygiV 3179385..3179867 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  NR351_RS15545 (NR351_15545) ygiW 3179920..3180312 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  NR351_RS15550 (NR351_15550) ciaR 3180464..3181123 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  NR351_RS15555 (NR351_15555) qseC 3181120..3182469 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  NR351_RS15560 (NR351_15560) ygiZ 3182515..3182847 (-) 333 WP_000917684.1 DUF2645 family protein -
  NR351_RS15565 (NR351_15565) mdaB 3183166..3183747 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  NR351_RS15570 (NR351_15570) ygiN 3183778..3184092 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  NR351_RS15575 (NR351_15575) parE 3184140..3186032 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=616229 NR351_RS15550 WP_001221495.1 3180464..3181123(+) (ciaR) [Escherichia coli strain JB41]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=616229 NR351_RS15550 WP_001221495.1 3180464..3181123(+) (ciaR) [Escherichia coli strain JB41]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361