Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LG336_RS20145 Genome accession   NZ_CP085225
Coordinates   4150637..4151719 (-) Length   360 a.a.
NCBI ID   WP_404329265.1    Uniprot ID   -
Organism   Mesobacillus maritimus strain ADH-29     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4145637..4156719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG336_RS20105 (LG336_19995) - 4146628..4147422 (+) 795 WP_404329259.1 YqhG family protein -
  LG336_RS20110 (LG336_20000) - 4147459..4147641 (-) 183 WP_404333237.1 YqzE family protein -
  LG336_RS20115 (LG336_20005) comGG 4147714..4148088 (-) 375 WP_404329260.1 competence type IV pilus minor pilin ComGG -
  LG336_RS20120 (LG336_20010) comGF 4148092..4148559 (-) 468 WP_404329261.1 competence type IV pilus minor pilin ComGF -
  LG336_RS20125 (LG336_20015) - 4148513..4148851 (-) 339 WP_404329262.1 phosphatase -
  LG336_RS20130 comGD 4148835..4149275 (-) 441 WP_404333238.1 competence type IV pilus minor pilin ComGD -
  LG336_RS20135 (LG336_20030) comGC 4149275..4149595 (-) 321 WP_404329263.1 competence type IV pilus major pilin ComGC -
  LG336_RS20140 (LG336_20035) comGB 4149619..4150653 (-) 1035 WP_404329264.1 competence type IV pilus assembly protein ComGB -
  LG336_RS20145 (LG336_20040) comGA 4150637..4151719 (-) 1083 WP_404329265.1 competence type IV pilus ATPase ComGA Machinery gene
  LG336_RS20150 (LG336_20045) - 4151967..4152668 (+) 702 WP_404329266.1 helix-turn-helix transcriptional regulator -
  LG336_RS20155 (LG336_20050) - 4152750..4152992 (+) 243 WP_404329267.1 DUF2626 domain-containing protein -
  LG336_RS20160 (LG336_20055) - 4153052..4154131 (-) 1080 WP_404329268.1 class I SAM-dependent methyltransferase -
  LG336_RS20165 (LG336_20060) - 4154373..4155008 (-) 636 WP_404329269.1 MBL fold metallo-hydrolase -
  LG336_RS20170 (LG336_20065) - 4155183..4155659 (+) 477 WP_404329270.1 hypothetical protein -
  LG336_RS20175 (LG336_20070) - 4155761..4155934 (+) 174 WP_404329271.1 DUF2759 domain-containing protein -
  LG336_RS20180 (LG336_20075) - 4155979..4156290 (-) 312 WP_404329272.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40352.82 Da        Isoelectric Point: 8.2390

>NTDB_id=616055 LG336_RS20145 WP_404329265.1 4150637..4151719(-) (comGA) [Mesobacillus maritimus strain ADH-29]
MFKIEQSIQALANRILTDALQKGASDIHIIPLGKNTVIKLRLGNQLLQRYHLEKDVCERLISHFKFISSMDIGDKRRPQS
GAHSYCYQGIEVGLRISTLPAAKLESMVIRLLPQQNQIPFYKLSLFPDTSNKLISLMKHAHGLVILTGPTGSGKSTTLYS
LLSEASQVINRHVITLEDPIEMQSDNMLQIQVNEKAGISYGSGLKAILRHDPDIIMVGEIRDQETAEIAVRAALTGHLVL
TTMHTRDAKGAIYRLLEFGINWLEIEQTLIAVTAQRLVELVCPYCESECPPYCYSAGRGKRGSIFEILSGSSLNAVLRES
KGMKEKIQYQTLRDALRKGIALGFIKEEEFKRWIMDESES

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=616055 LG336_RS20145 WP_404329265.1 4150637..4151719(-) (comGA) [Mesobacillus maritimus strain ADH-29]
GTGTTTAAAATTGAACAATCTATTCAAGCATTAGCAAATCGAATTTTGACAGATGCATTGCAAAAAGGCGCTTCTGACAT
CCATATCATCCCTTTAGGGAAAAATACGGTAATAAAGTTACGCCTCGGCAACCAACTCCTGCAAAGGTATCATCTCGAAA
AGGACGTATGCGAACGACTCATATCGCATTTTAAATTTATTTCTTCCATGGATATTGGCGATAAAAGGCGTCCGCAAAGT
GGAGCGCACTCCTACTGTTATCAAGGTATTGAGGTTGGTCTAAGAATTTCAACCCTTCCTGCAGCCAAACTTGAAAGTAT
GGTCATCCGCTTACTACCCCAGCAAAACCAAATTCCCTTTTACAAACTTTCTCTATTCCCAGACACATCAAATAAACTCA
TTTCTCTTATGAAGCATGCCCATGGATTGGTTATCCTCACCGGTCCAACCGGAAGTGGAAAGTCAACCACTTTGTATTCT
CTTCTTTCAGAAGCCTCACAGGTCATCAACAGACATGTCATAACGCTTGAAGACCCCATTGAAATGCAGAGCGATAACAT
GCTCCAAATTCAGGTGAACGAAAAGGCTGGAATCTCTTATGGTTCCGGATTAAAAGCCATTCTTCGTCATGACCCGGATA
TTATTATGGTTGGAGAAATCCGTGACCAAGAAACAGCAGAAATTGCTGTGCGCGCGGCACTAACCGGTCATCTTGTATTG
ACAACGATGCATACTCGAGATGCAAAGGGAGCGATTTATCGTCTTCTAGAATTCGGAATTAACTGGTTAGAAATTGAACA
GACCTTAATTGCCGTAACCGCACAGCGCTTAGTGGAGCTAGTATGCCCTTATTGTGAAAGTGAATGTCCACCCTATTGCT
ATTCAGCAGGTCGTGGAAAAAGGGGGAGTATATTTGAAATTTTGAGTGGTAGTTCATTAAATGCTGTACTGAGAGAATCG
AAGGGGATGAAGGAAAAGATTCAGTATCAAACGTTAAGGGATGCCTTGAGGAAGGGGATTGCACTAGGATTTATTAAGGA
GGAAGAGTTTAAGAGGTGGATTATGGATGAATCAGAAAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

54.237

98.333

0.533
  comGA Staphylococcus aureus MW2

37.714

97.222

0.367
  comGA Staphylococcus aureus N315

37.714

97.222

0.367
  pilB Glaesserella parasuis strain SC1401

37.822

96.944

0.367
  pilB Haemophilus influenzae 86-028NP

37.322

97.5

0.364