Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LF296_RS18440 Genome accession   NZ_CP085083
Coordinates   3921725..3922915 (+) Length   396 a.a.
NCBI ID   WP_272655156.1    Uniprot ID   A0AAJ6P595
Organism   Acinetobacter vivianii strain KJ-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3916725..3927915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF296_RS18415 (LF296_18415) - 3917275..3917646 (-) 372 WP_272655154.1 hypothetical protein -
  LF296_RS18420 (LF296_18420) guaD 3917967..3919283 (+) 1317 WP_272655155.1 guanine deaminase -
  LF296_RS18425 (LF296_18425) hpt 3919509..3920036 (+) 528 WP_004776485.1 hypoxanthine phosphoribosyltransferase -
  LF296_RS18430 (LF296_18430) - 3920288..3920539 (+) 252 WP_004776487.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  LF296_RS18435 (LF296_18435) - 3920596..3921294 (-) 699 WP_005260353.1 DsbC family protein -
  LF296_RS18440 (LF296_18440) pilU 3921725..3922915 (+) 1191 WP_272655156.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LF296_RS18445 (LF296_18445) mnmE 3922969..3924324 (-) 1356 WP_272655157.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  LF296_RS18450 (LF296_18450) yidC 3924439..3926196 (-) 1758 WP_004776493.1 membrane protein insertase YidC -
  LF296_RS18455 (LF296_18455) yidD 3926202..3926522 (-) 321 WP_004660550.1 membrane protein insertion efficiency factor YidD -
  LF296_RS18460 (LF296_18460) rnpA 3926532..3926927 (-) 396 WP_005260345.1 ribonuclease P protein component -
  LF296_RS18465 (LF296_18465) rpmH 3926955..3927089 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 44801.44 Da        Isoelectric Point: 6.4466

>NTDB_id=615140 LF296_RS18440 WP_272655156.1 3921725..3922915(+) (pilU) [Acinetobacter vivianii strain KJ-1]
MYSAELLEEAKTFMFHMLTKVVEYGVSDLFITADFPPSIKLQGLMRPLGQQELTADKTKLFAYSLMNEKQRQDFENEWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWQHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKGIISQRLIRTQDGKGRRAAIEILLNTPLISDNILKGQFHALKEIMSKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKATRQEGAPVAMMTALNVVAEPKAEEEQTAEEKE

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=615140 LF296_RS18440 WP_272655156.1 3921725..3922915(+) (pilU) [Acinetobacter vivianii strain KJ-1]
ATGTATAGCGCAGAATTACTCGAAGAAGCCAAAACATTTATGTTTCATATGCTGACCAAAGTAGTGGAATACGGTGTTTC
AGATTTATTTATCACCGCAGATTTTCCACCTAGCATTAAATTACAAGGACTTATGCGTCCACTCGGGCAACAGGAACTGA
CTGCCGATAAAACCAAACTCTTCGCGTATAGCTTAATGAATGAAAAACAGCGCCAAGATTTTGAAAATGAATGGGAATGT
AATTTTGCCATCAATGTTCCCAATGTTTCACGTTTTCGTGTCAATGTATTTAAACAGCAGCTACAGATTGGTATGGTGAT
TCGTACCATTACCTCGGAAATTCCAACCTTTCAGAAGCTAAAGCTGCCTGAATCGCTGAAACATGTGATTATGGAAAAAC
GTGGTCTGGTTCTGGTTGTCGGTGGTACAGGCTCAGGCAAATCAACCTCTTTGGCGGCCATGATTGATCACCGCAATGAA
AACTCAGCGGGGCATATTATTACCGTTGAAGATCCGGTTGAGTACGTGCATAAACACAAAAAATCCATGATTACCCATCG
TGAAGTCGGTGTTGATTGTCATTCATGGCAGCATGCCTTGAAAAATACCTTACGTCAGGCGCCCGATGTGATTCTGATTG
GGGAAATCCGTGATACGGAGACCATGGAACATGCGATTGCTTTTGCGGAAACAGGACATCTTTGTCTCGGTACCTTACAT
GCCAACAATGCCAATCAGGCGCTAGACCGGATTATCAACTTCTTTCCAGAAGAACGTCGTAACCAGTTACTCATGGATCT
TTCATCCAATATGAAAGGCATTATTTCTCAACGTTTGATACGAACGCAGGATGGCAAAGGTCGTCGGGCAGCGATTGAAA
TTTTGCTCAATACGCCATTGATTTCAGATAATATTTTAAAAGGCCAGTTCCACGCACTCAAAGAGATTATGAGCAAATCG
CGAGAGCTGGGGATGCAAACCTTCGATCAGGCTTTATTCGATCTCTATAACGAAGGTGCCATCAGCTATGATGAAGCCTT
ACGTAACGCCGATTCAGTCAATGAATTACGTTTACAGATCAAGCTCAAAGCTACACGTCAGGAAGGCGCACCGGTTGCCA
TGATGACTGCATTAAACGTCGTAGCAGAACCAAAAGCTGAGGAAGAGCAAACCGCTGAAGAGAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(88-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.195

87.879

0.52
  pilU Acinetobacter baylyi ADP1

56.484

87.626

0.495
  pilU Vibrio cholerae strain A1552

52.89

87.374

0.462