Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LG282_RS15940 Genome accession   NZ_CP085020
Coordinates   3110588..3111658 (-) Length   356 a.a.
NCBI ID   WP_404292305.1    Uniprot ID   -
Organism   Cytobacillus kochii strain SLM-180     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3105588..3116658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG282_RS15900 (LG282_15785) - 3106479..3107276 (+) 798 WP_224935993.1 YqhG family protein -
  LG282_RS15905 (LG282_15790) - 3107471..3107662 (-) 192 WP_095372590.1 YqzE family protein -
  LG282_RS15910 (LG282_15795) comGG 3107721..3108116 (-) 396 WP_404292297.1 competence type IV pilus minor pilin ComGG -
  LG282_RS15915 (LG282_15800) comGF 3108097..3108555 (-) 459 WP_404292299.1 competence type IV pilus minor pilin ComGF -
  LG282_RS15920 (LG282_15805) - 3108542..3108841 (-) 300 WP_224935990.1 hypothetical protein -
  LG282_RS15925 (LG282_15810) comGD 3108825..3109316 (-) 492 WP_404292301.1 competence type IV pilus minor pilin ComGD -
  LG282_RS15930 (LG282_15815) comGC 3109253..3109570 (-) 318 WP_224935988.1 competence type IV pilus major pilin ComGC -
  LG282_RS15935 (LG282_15820) comGB 3109570..3110601 (-) 1032 WP_404292303.1 competence type IV pilus assembly protein ComGB -
  LG282_RS15940 (LG282_15825) comGA 3110588..3111658 (-) 1071 WP_404292305.1 competence type IV pilus ATPase ComGA Machinery gene
  LG282_RS15945 (LG282_15830) - 3111922..3112611 (+) 690 WP_095372582.1 metalloregulator ArsR/SmtB family transcription factor -
  LG282_RS15950 (LG282_15835) - 3112696..3112941 (+) 246 WP_095372581.1 DUF2626 domain-containing protein -
  LG282_RS15955 (LG282_15840) - 3113003..3114064 (-) 1062 WP_224935985.1 SAM-dependent methyltransferase -
  LG282_RS15960 (LG282_15845) - 3114064..3114702 (-) 639 WP_341106705.1 MBL fold metallo-hydrolase -
  LG282_RS15965 (LG282_15850) - 3114960..3115133 (+) 174 WP_095372578.1 DUF2759 domain-containing protein -
  LG282_RS15970 (LG282_15855) - 3115185..3115505 (-) 321 WP_251161982.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40074.32 Da        Isoelectric Point: 9.5859

>NTDB_id=614620 LG282_RS15940 WP_404292305.1 3110588..3111658(-) (comGA) [Cytobacillus kochii strain SLM-180]
MVSMQRMATLILREAVKTEVTDIHFVPRKNHTIIYFRYGKRLVPKHYIPHYESDRLISHFKFIASMDIGEKRKPQNGAYS
TDIEGYDIGLRFSTLPSQYNESLVIRILPQSSHISSHNVSLFPTMSRKILAMLKHAHGMIILTGPTGSGKTSTLYSLLND
NSNLFNRSIITLEDPIEKKTDSLLQVEVNEKAGVTYATGLKAILRHDPDIIMVGEIRDAQTAKIAVRAALTGHLVLTTMH
TRNAVGAIHRLVEFGVAWKQIEQTLVAITAQRLVELTCPFCEGECTDFCYSCGRLKRAGVFELLSGRELNQALKAAKSSQ
NNYEPYFSLRSALKKGVALGYVKESEFNRWILDEKS

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=614620 LG282_RS15940 WP_404292305.1 3110588..3111658(-) (comGA) [Cytobacillus kochii strain SLM-180]
TTGGTCTCAATGCAACGCATGGCTACCTTAATTTTAAGGGAAGCTGTGAAAACAGAAGTAACAGATATTCATTTTGTCCC
CAGAAAGAATCACACAATCATTTATTTCCGCTATGGCAAGCGCCTTGTCCCTAAACATTACATCCCTCATTATGAATCTG
ACCGTTTAATTTCTCATTTCAAGTTTATTGCATCCATGGATATTGGTGAAAAACGAAAGCCACAAAATGGCGCCTATTCT
ACAGATATTGAAGGATATGATATAGGCTTACGTTTCTCCACATTGCCCTCTCAATACAATGAAAGTCTTGTCATCCGTAT
CCTGCCACAATCGTCTCATATTTCTTCTCACAATGTTTCACTATTTCCAACGATGTCCCGAAAAATCCTTGCTATGCTTA
AACATGCGCACGGGATGATCATACTAACTGGTCCGACCGGTAGTGGAAAAACCTCAACCTTATATAGTCTGCTAAATGAC
AATTCCAACCTATTCAATCGAAGTATTATCACTTTAGAAGATCCGATTGAGAAAAAGACTGACTCCTTACTGCAAGTCGA
AGTAAATGAAAAGGCAGGTGTAACATATGCTACTGGTTTGAAAGCTATCCTTAGGCACGACCCGGACATAATTATGGTTG
GGGAAATACGCGACGCCCAAACAGCTAAAATAGCTGTGAGAGCAGCATTAACTGGGCATTTAGTATTAACGACGATGCAT
ACGAGAAATGCTGTTGGAGCCATACATCGTTTAGTGGAGTTTGGGGTAGCGTGGAAACAAATAGAACAAACGTTAGTAGC
CATAACAGCACAGAGGCTTGTAGAATTAACCTGCCCATTTTGTGAAGGAGAATGTACCGATTTCTGTTATAGCTGTGGCA
GGTTAAAAAGAGCTGGTGTGTTTGAATTATTGAGTGGAAGGGAATTGAATCAAGCTTTGAAAGCGGCGAAAAGTAGTCAG
AATAATTACGAACCATATTTTTCGCTCAGGTCAGCTTTGAAAAAAGGGGTAGCCTTAGGGTATGTAAAAGAAAGTGAGTT
TAATCGGTGGATTTTAGATGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

53.221

100

0.534
  pilB Glaesserella parasuis strain SC1401

39.104

94.101

0.368
  pilB Legionella pneumophila strain ERS1305867

34.392

100

0.365
  pilB Acinetobacter baumannii D1279779

34.121

100

0.365