NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	NP446_RS23545	Genome accession	NZ_CP101912
Coordinates	4990025..4990696 (-)	Length	223 a.a.
NCBI ID	WP_003085524.1	Uniprot ID	A0A0H2Z6L5
Organism	Pseudomonas aeruginosa strain ATCC 27853
Function	repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 4985025..4995696

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP446_RS23520 (NP446_23520)	-	4985798..4986250 (-)	453	WP_016851963.1	protein YgfX	-
NP446_RS23525 (NP446_23525)	-	4986234..4986488 (-)	255	WP_003085532.1	succinate dehydrogenase assembly factor 2	-
NP446_RS23530 (NP446_23530)	-	4986761..4987705 (+)	945	WP_003101947.1	folate-binding protein YgfZ	-
NP446_RS23535 (NP446_23535)	-	4987806..4988642 (+)	837	WP_003114179.1	HDOD domain-containing protein	-
NP446_RS23540 (NP446_23540)	-	4988650..4990032 (-)	1383	WP_012614480.1	sensor histidine kinase	-
NP446_RS23545 (NP446_23545)	ciaR	4990025..4990696 (-)	672	WP_003085524.1	response regulator	Regulator
NP446_RS23550 (NP446_23550)	-	4990907..4992190 (+)	1284	WP_016851964.1	OprD family porin	-
NP446_RS23555 (NP446_23555)	-	4992220..4993203 (+)	984	WP_003085519.1	tripartite tricarboxylate transporter substrate binding protein	-
NP446_RS23560 (NP446_23560)	-	4993252..4993722 (+)	471	WP_003101933.1	tripartite tricarboxylate transporter TctB family protein	-
NP446_RS23565 (NP446_23565)	-	4993733..4995250 (+)	1518	WP_003116509.1	tripartite tricarboxylate transporter permease	-

Sequence

Protein

Download Length: 223 a.a. Molecular weight: 24431.24 Da Isoelectric Point: 5.1881

                                >NTDB_id=612358 NP446_RS23545 WP_003085524.1 4990025..4990696(-) (ciaR) [Pseudomonas aeruginosa strain ATCC 27853]

MRILLVEDHPQLAESVVQALKGAGWTVDLLQDGVAADLALASEEYALAILDVGLPRMDGFEVLARLRGRGKTLPVLMLTA

RGEVKDRVHGLNLGADDYLAKPFELSELEARVKALLRRSVLGGEQLQRCGALVYDLGTRRFSLDEQPLTLTSREQAVLEA

MIARPGRVMSKEQLAAQVFGLDEEASADAIEIYVHRLRKKLEGGAVRIVTFRGLGYLLEAQGD

Nucleotide

Download Length: 672 bp

                                >NTDB_id=612358 NP446_RS23545 WP_003085524.1 4990025..4990696(-) (ciaR) [Pseudomonas aeruginosa strain ATCC 27853]

ATGCGCATCCTTCTGGTGGAAGATCATCCGCAGCTCGCCGAAAGCGTGGTCCAGGCGCTCAAGGGCGCCGGCTGGACGGT

AGACCTGCTGCAGGACGGGGTGGCCGCCGACCTGGCCCTGGCCAGCGAGGAGTACGCCCTGGCGATCCTCGACGTCGGCC

TGCCGCGGATGGACGGCTTCGAGGTGCTGGCGCGCCTGCGCGGGCGCGGCAAGACCCTGCCGGTGCTGATGCTGACTGCG

CGCGGCGAGGTGAAGGACCGGGTGCACGGCCTCAATCTCGGCGCCGACGACTACCTGGCAAAGCCTTTCGAACTGTCCGA

GCTGGAGGCGCGGGTCAAGGCCCTGCTGCGCCGCAGCGTGCTCGGTGGCGAACAGTTGCAGCGCTGCGGCGCCCTGGTCT

ACGACCTCGGCACCCGGCGCTTCAGCCTCGACGAGCAGCCGCTGACCCTGACCTCGCGGGAACAGGCGGTGCTGGAAGCG

ATGATCGCCCGTCCGGGGCGGGTGATGAGCAAGGAGCAACTGGCCGCCCAGGTCTTCGGCCTGGACGAGGAGGCCAGCGC

CGACGCCATCGAGATCTACGTCCACCGCCTGCGCAAGAAGCTGGAAGGCGGCGCCGTGCGCATCGTCACCTTCCGCGGCC

TCGGCTACCTGCTGGAGGCGCAGGGTGACTGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(146-218)

Response regulator receiver domain (Response_reg)

(3-112)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H2Z6L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus pneumoniae Rx1	37.668	100	0.377
ciaR	Streptococcus pneumoniae D39	37.668	100	0.377
ciaR	Streptococcus pneumoniae R6	37.668	100	0.377
ciaR	Streptococcus pneumoniae TIGR4	37.668	100	0.377
covR	Lactococcus lactis subsp. lactis strain DGCC12653	37.668	100	0.377

Detailed information NTG0612358