Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LDO17_RS16985 Genome accession   NZ_CP084539
Coordinates   3583834..3584910 (-) Length   358 a.a.
NCBI ID   WP_105718781.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans strain Ant232     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3578834..3589910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO17_RS16945 - 3579726..3580523 (+) 798 WP_048688306.1 YqhG family protein -
  LDO17_RS16950 - 3580568..3580753 (-) 186 WP_048688303.1 YqzE family protein -
  LDO17_RS16955 comGG 3580846..3581232 (-) 387 WP_048688300.1 competence type IV pilus minor pilin ComGG -
  LDO17_RS16960 comGF 3581225..3581704 (-) 480 WP_081088666.1 competence type IV pilus minor pilin ComGF -
  LDO17_RS16965 - 3581652..3581990 (-) 339 WP_048688295.1 hypothetical protein -
  LDO17_RS16970 comGD 3581977..3582420 (-) 444 WP_048688291.1 competence type IV pilus minor pilin ComGD -
  LDO17_RS16975 comGC 3582428..3582739 (-) 312 WP_048688337.1 competence type IV pilus major pilin ComGC -
  LDO17_RS16980 comGB 3582816..3583850 (-) 1035 WP_065421218.1 competence type IV pilus assembly protein ComGB -
  LDO17_RS16985 comGA 3583834..3584910 (-) 1077 WP_105718781.1 competence type IV pilus ATPase ComGA Machinery gene
  LDO17_RS16990 - 3585015..3585395 (-) 381 WP_048682187.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LDO17_RS16995 - 3585611..3586312 (+) 702 WP_048682184.1 helix-turn-helix domain-containing protein -
  LDO17_RS17000 - 3586398..3586640 (+) 243 WP_034308393.1 DUF2626 domain-containing protein -
  LDO17_RS17005 - 3586693..3587793 (-) 1101 WP_048682197.1 SAM-dependent methyltransferase -
  LDO17_RS17010 - 3588101..3588727 (-) 627 WP_048682180.1 MBL fold metallo-hydrolase -
  LDO17_RS17015 - 3588961..3589134 (+) 174 WP_034308396.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39778.43 Da        Isoelectric Point: 8.4720

>NTDB_id=611769 LDO17_RS16985 WP_105718781.1 3583834..3584910(-) (comGA) [Peribacillus frigoritolerans strain Ant232]
MISIEKTAEKILTRAIVESASDIHIFFRGEGPLIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQTLIGVTAQRLVELRCLTCKGDCALACKMTARNKRASVYELLYGKSLAEVLRIMGDEK
GKATVSYRQLKDEIGKAVAMGYVDAQEYERLVYHETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=611769 LDO17_RS16985 WP_105718781.1 3583834..3584910(-) (comGA) [Peribacillus frigoritolerans strain Ant232]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTAACCCGTGCCATCGTGGAGTCGGCATCGGATATCCACATTTTTTT
TCGCGGAGAGGGGCCTCTCATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACATTATCATTTTTTGAAGCTG
AAAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGCGCCATCACC
ATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTGCACTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACTTTATATTCCCTGCTTCACCAC
GCCCATGAGATGATCAATCGAAATATCATTACACTTGAAGACCCCATTGAAAATGTATCCGAAAAGGTATTGCAAGTCCA
AATCAATGAAAAGGCGGGCATTACGTATTCCGTCGGCCTAAAAGCTGTTCTGAGGCATGACCCGGACGTGATCATGGTTG
GGGAAGTCAGGGATGCAGAAACTGCCAAAATCGCAGTCCGTGCCGCATTAACCGGTCATTTGATACTAACGACCATGCAT
ACGAGGGATGCCCAGGGTGCCATCTCCAGATTACTGGAATTTGGTGTCAGCTTACTTGAGGTTGAACAGACTTTGATTGG
CGTGACAGCGCAGCGGCTGGTTGAATTGCGATGTCTTACGTGTAAAGGGGACTGTGCTTTAGCTTGCAAAATGACTGCGA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGTCTGGCTGAAGTTCTCCGGATAATGGGAGATGAAAAA
GGAAAGGCAACGGTCAGCTACCGCCAATTAAAGGATGAAATCGGAAAAGCGGTTGCGATGGGCTATGTGGATGCTCAGGA
ATATGAACGGCTGGTATACCATGAAACCAAAAAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

38.506

97.207

0.374
  pilB Haemophilus influenzae Rd KW20

37.356

97.207

0.363