Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LA387_RS03575 Genome accession   NZ_CP084377
Coordinates   705287..706543 (+) Length   418 a.a.
NCBI ID   WP_004257939.1    Uniprot ID   -
Organism   Lactococcus garvieae strain Lg-Granada     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 700287..711543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA387_RS03550 glyQ 700441..701409 (-) 969 WP_004257927.1 glycine--tRNA ligase subunit alpha -
  LA387_RS03555 - 701423..702202 (-) 780 WP_004257929.1 formate/nitrite transporter family protein -
  LA387_RS03560 - 702521..703543 (+) 1023 WP_004257931.1 alpha/beta hydrolase -
  LA387_RS03565 - 703573..704595 (-) 1023 WP_004257934.1 DUF475 domain-containing protein -
  LA387_RS03570 - 704609..705232 (-) 624 WP_004257938.1 YigZ family protein -
  LA387_RS03575 comFA 705287..706543 (+) 1257 WP_004257939.1 DEAD/DEAH box helicase Machinery gene
  LA387_RS03580 - 706540..707184 (+) 645 WP_004257941.1 ComF family protein -
  LA387_RS03585 - 707187..707648 (-) 462 WP_004257944.1 diacylglycerol kinase family protein -
  LA387_RS03590 ybeY 707632..708120 (-) 489 WP_004257946.1 rRNA maturation RNase YbeY -
  LA387_RS03595 - 708130..708603 (-) 474 WP_004257950.1 NUDIX hydrolase -
  LA387_RS03600 - 708605..709564 (-) 960 WP_004257953.1 PhoH family protein -
  LA387_RS03605 - 709682..710788 (-) 1107 WP_004257955.1 alanine dehydrogenase -
  LA387_RS03610 rpmA 711140..711424 (-) 285 WP_004257956.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47861.62 Da        Isoelectric Point: 9.9268

>NTDB_id=610837 LA387_RS03575 WP_004257939.1 705287..706543(+) (comFA) [Lactococcus garvieae strain Lg-Granada]
MDELYGRLLLQKELTKLPDKAILFDGMQDVSQTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FSAASFLNWQGKLTASQQHVSDSLVNLHQQQKTVLVQAVTGAGKTEMIYQSINNTLKKGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATAHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFALTQARKPSSSLIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRQGEKLCQDLQSNFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=610837 LA387_RS03575 WP_004257939.1 705287..706543(+) (comFA) [Lactococcus garvieae strain Lg-Granada]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAATACTTTTTGATGGGAT
GCAAGATGTTTCCCAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATTAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCTCATTGTATTCAAATGGGACGTGTTCGCTCGGACGAAAAACTTTACCACCTTCCACAGGAGGAT
TTTTCAGCAGCTTCTTTTCTCAACTGGCAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAATTT
GCATCAGCAACAAAAAACAGTACTGGTACAGGCGGTTACTGGTGCAGGAAAAACTGAAATGATTTATCAGAGCATTAATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGCTCACCACTCGTAATTGCAACGGC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTTGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGCGCTGACTCAAGCCCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAATTAGGACAAATCGAGAAGCTACAGCTCCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAGTCTGGTTTTCCCTTACTTATCTTTGTCC
CTGAAATAAGACAGGGAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTCGCGTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGCTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATTTAGGAAAAACTACAGCG
ATGTTGGAAGCCTATAAAAATATCAGAAATATGAATAAAGCAGGAGGGTTCTTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(92-236)

Helicase conserved C-terminal domain (Helicase_C)

(274-382)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.143

95.455

0.545
  comFA/cflA Streptococcus mitis SK321

51.878

100

0.529
  comFA/cflA Streptococcus pneumoniae TIGR4

51.878

100

0.529
  comFA/cflA Streptococcus pneumoniae Rx1

51.878

100

0.529
  comFA/cflA Streptococcus pneumoniae D39

51.878

100

0.529
  comFA/cflA Streptococcus pneumoniae R6

51.878

100

0.529
  comFA/cflA Streptococcus mitis NCTC 12261

50.939

100

0.519
  comFA Bacillus subtilis subsp. subtilis str. 168

38.791

94.976

0.368
  comFA Latilactobacillus sakei subsp. sakei 23K

38.287

94.976

0.364