Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   NML18_RS03455 Genome accession   NZ_CP101269
Coordinates   707151..707810 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain EC21Z-048     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 702151..712810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML18_RS03430 (NML18_03435) parE 702242..704134 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  NML18_RS03435 (NML18_03440) ygiN 704182..704496 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  NML18_RS03440 (NML18_03445) mdaB 704527..705108 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  NML18_RS03445 (NML18_03450) ygiZ 705427..705759 (+) 333 WP_000912120.1 DUF2645 family protein -
  NML18_RS03450 (NML18_03455) qseC 705805..707154 (-) 1350 WP_254837359.1 quorum sensing histidine kinase QseC -
  NML18_RS03455 (NML18_03460) ciaR 707151..707810 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  NML18_RS03460 (NML18_03465) ygiW 707962..708354 (+) 393 WP_104725572.1 OB fold stress tolerance protein YgiW -
  NML18_RS03465 (NML18_03470) ygiV 708407..708889 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  NML18_RS03470 (NML18_03475) parC 709435..711693 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  NML18_RS03475 (NML18_03480) plsC 711926..712663 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=609787 NML18_RS03455 WP_001221502.1 707151..707810(-) (ciaR) [Escherichia coli strain EC21Z-048]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=609787 NML18_RS03455 WP_001221502.1 707151..707810(-) (ciaR) [Escherichia coli strain EC21Z-048]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365