NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	NML20_RS03590	Genome accession	NZ_CP101257
Coordinates	740886..741545 (-)	Length	219 a.a.
NCBI ID	WP_001221502.1	Uniprot ID	B7LGG8
Organism	Escherichia coli strain EC21Z-078
Function	repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 735886..746545

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NML20_RS03560 (NML20_03560)	ygiN	736787..737101 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
NML20_RS03565 (NML20_03565)	mdaB	737132..737713 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
NML20_RS03570 (NML20_03570)	-	737963..738442 (+)	480	WP_000065332.1	Hcp family type VI secretion system effector	-
NML20_RS03575 (NML20_03575)	-	738445..739155 (+)	711	WP_000834030.1	hypothetical protein	-
NML20_RS03580 (NML20_03580)	ygiZ	739162..739494 (+)	333	WP_000914690.1	DUF2645 family protein	-
NML20_RS03585 (NML20_03585)	qseC	739540..740889 (-)	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
NML20_RS03590 (NML20_03590)	ciaR	740886..741545 (-)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
NML20_RS03595 (NML20_03595)	ygiW	741697..742089 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
NML20_RS03600 (NML20_03600)	ygiV	742142..742624 (+)	483	WP_000183505.1	GyrI-like domain-containing protein	-
NML20_RS03605 (NML20_03605)	mqsR	742829..743125 (+)	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	-
NML20_RS03610 (NML20_03610)	mqsA	743127..743522 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
NML20_RS03615 (NML20_03615)	ygiS	743655..745262 (+)	1608	WP_001375094.1	ABC transporter substrate-binding protein	-

Sequence

Protein

Download Length: 219 a.a. Molecular weight: 24745.66 Da Isoelectric Point: 6.6543

                                >NTDB_id=609629 NML20_RS03590 WP_001221502.1 740886..741545(-) (ciaR) [Escherichia coli strain EC21Z-078]

MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA

RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL

LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

Download Length: 660 bp

                                >NTDB_id=609629 NML20_RS03590 WP_001221502.1 740886..741545(-) (ciaR) [Escherichia coli strain EC21Z-078]

ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT

CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT

TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG

CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA

AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC

TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA

CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG

TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT

ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365

Detailed information NTG0609629