NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	NML21_RS03585	Genome accession	NZ_CP101251
Coordinates	740772..741431 (-)	Length	219 a.a.
NCBI ID	WP_001221502.1	Uniprot ID	B7LGG8
Organism	Escherichia coli strain EC21Z-083
Function	repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 735772..746431

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NML21_RS03555 (NML21_03565)	ygiN	736673..736987 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
NML21_RS03560 (NML21_03570)	mdaB	737018..737599 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
NML21_RS03565 (NML21_03575)	-	737849..738328 (+)	480	WP_000065332.1	Hcp family type VI secretion system effector	-
NML21_RS03570 (NML21_03580)	-	738331..739041 (+)	711	WP_000834030.1	hypothetical protein	-
NML21_RS03575 (NML21_03585)	ygiZ	739048..739380 (+)	333	WP_000914690.1	DUF2645 family protein	-
NML21_RS03580 (NML21_03590)	qseC	739426..740775 (-)	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
NML21_RS03585 (NML21_03595)	ciaR	740772..741431 (-)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
NML21_RS03590 (NML21_03600)	ygiW	741583..741975 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
NML21_RS03595 (NML21_03605)	ygiV	742028..742510 (+)	483	WP_000183505.1	GyrI-like domain-containing protein	-
NML21_RS03600 (NML21_03610)	mqsR	742715..743011 (+)	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	-
NML21_RS03605 (NML21_03615)	mqsA	743013..743408 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
NML21_RS03610 (NML21_03620)	ygiS	743541..745148 (+)	1608	WP_001375094.1	ABC transporter substrate-binding protein	-

Sequence

Protein

Download Length: 219 a.a. Molecular weight: 24745.66 Da Isoelectric Point: 6.6543

                                >NTDB_id=609550 NML21_RS03585 WP_001221502.1 740772..741431(-) (ciaR) [Escherichia coli strain EC21Z-083]

MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA

RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL

LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

Download Length: 660 bp

                                >NTDB_id=609550 NML21_RS03585 WP_001221502.1 740772..741431(-) (ciaR) [Escherichia coli strain EC21Z-083]

ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT

CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT

TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG

CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA

AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC

TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA

CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG

TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT

ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365

Detailed information NTG0609550