Detailed information NTG0609066

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: NML27_RS03645
• Genome accession: NZ_CP101197
• Coordinates: 757300..757959 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain EC21Z-151
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 752300..762959

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NML27_RS03615 (NML27_03615)	-	752416..753363 (+)	948	WP_001305988.1	iron-siderophore ABC transporter substrate-binding protein	-
NML27_RS03620 (NML27_03620)	-	753360..754247 (+)	888	WP_000614953.1	MurR/RpiR family transcriptional regulator	-
NML27_RS03625 (NML27_03625)	ygiN	754292..754606 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
NML27_RS03630 (NML27_03630)	mdaB	754637..755218 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
NML27_RS03635 (NML27_03635)	ygiZ	755576..755905 (+)	330	WP_001551659.1	DUF2645 family protein	-
NML27_RS03640 (NML27_03640)	qseC	755954..757303 (-)	1350	WP_001551658.1	quorum sensing histidine kinase QseC	-
NML27_RS03645 (NML27_03645)	ciaR	757300..757959 (-)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
NML27_RS03650 (NML27_03650)	ygiW	758111..758503 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
NML27_RS03655 (NML27_03655)	ygiV	758556..759038 (+)	483	WP_000183505.1	GyrI-like domain-containing protein	-
NML27_RS03660 (NML27_03660)	mqsR	759243..759539 (+)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
NML27_RS03665 (NML27_03665)	mqsA	759541..759936 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
NML27_RS03670 (NML27_03670)	ygiS	760069..761676 (+)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=609066 NML27_RS03645 WP_001221493.1 757300..757959(-) (ciaR) [Escherichia coli strain EC21Z-151]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=609066 NML27_RS03645 WP_001221493.1 757300..757959(-) (ciaR) [Escherichia coli strain EC21Z-151]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365