Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LCH16_RS12460 Genome accession   NZ_CP083943
Coordinates   2583665..2584735 (-) Length   356 a.a.
NCBI ID   WP_213390994.1    Uniprot ID   -
Organism   Bacillus velezensis strain CSUSB3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2578665..2589735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH16_RS12410 (LCH16_12410) sipW 2578725..2579309 (-) 585 WP_012117977.1 signal peptidase I SipW -
  LCH16_RS12415 (LCH16_12415) tapA 2579281..2579952 (-) 672 WP_165625718.1 amyloid fiber anchoring/assembly protein TapA -
  LCH16_RS12420 (LCH16_12420) - 2580211..2580540 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  LCH16_RS12425 (LCH16_12425) - 2580580..2580759 (-) 180 WP_003153093.1 YqzE family protein -
  LCH16_RS12430 (LCH16_12430) comGG 2580816..2581193 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  LCH16_RS12435 (LCH16_12435) comGF 2581194..2581589 (-) 396 WP_003153090.1 competence type IV pilus minor pilin ComGF -
  LCH16_RS12440 (LCH16_12440) comGE 2581603..2581917 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  LCH16_RS12445 (LCH16_12445) comGD 2581901..2582338 (-) 438 WP_224462754.1 competence type IV pilus minor pilin ComGD Machinery gene
  LCH16_RS12450 (LCH16_12450) comGC 2582328..2582636 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  LCH16_RS12455 (LCH16_12455) comGB 2582641..2583678 (-) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  LCH16_RS12460 (LCH16_12460) comGA 2583665..2584735 (-) 1071 WP_213390994.1 competence type IV pilus ATPase ComGA Machinery gene
  LCH16_RS12465 (LCH16_12465) - 2584927..2585877 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  LCH16_RS12470 (LCH16_12470) - 2586023..2587324 (+) 1302 WP_014305416.1 hemolysin family protein -
  LCH16_RS12475 (LCH16_12475) - 2587362..2588192 (-) 831 WP_039254482.1 STAS domain-containing protein -
  LCH16_RS12485 (LCH16_12485) - 2588399..2588779 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LCH16_RS12490 (LCH16_12490) - 2589016..2589261 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40519.81 Da        Isoelectric Point: 9.4729

>NTDB_id=608685 LCH16_RS12460 WP_213390994.1 2583665..2584735(-) (comGA) [Bacillus velezensis strain CSUSB3]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPSLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=608685 LCH16_RS12460 WP_213390994.1 2583665..2584735(-) (comGA) [Bacillus velezensis strain CSUSB3]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCTCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATACTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGTGCGGCTATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGTGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.506

97.753

0.376
  pilF Thermus thermophilus HB27

37.892

98.596

0.374