Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   NMD25_RS03655 Genome accession   NZ_CP101010
Coordinates   751999..752658 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain ET76     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 746999..757658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD25_RS03630 (NMD25_03615) parE 747090..748982 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  NMD25_RS03635 (NMD25_03620) ygiN 749030..749344 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  NMD25_RS03640 (NMD25_03625) mdaB 749375..749956 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  NMD25_RS03645 (NMD25_03630) ygiZ 750275..750607 (+) 333 WP_000917684.1 DUF2645 family protein -
  NMD25_RS03650 (NMD25_03635) qseC 750653..752002 (-) 1350 WP_001618857.1 quorum sensing histidine kinase QseC -
  NMD25_RS03655 (NMD25_03640) ciaR 751999..752658 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  NMD25_RS03660 (NMD25_03645) ygiW 752810..753202 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  NMD25_RS03665 (NMD25_03650) ygiV 753255..753737 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  NMD25_RS03670 (NMD25_03655) mqsR 753942..754238 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  NMD25_RS03675 (NMD25_03660) mqsA 754240..754635 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  NMD25_RS03680 (NMD25_03665) ygiS 754768..756375 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=608647 NMD25_RS03655 WP_001221493.1 751999..752658(-) (ciaR) [Escherichia coli strain ET76]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=608647 NMD25_RS03655 WP_001221493.1 751999..752658(-) (ciaR) [Escherichia coli strain ET76]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365