Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JF568_RS02930 Genome accession   NZ_CP083931
Coordinates   588614..590686 (-) Length   690 a.a.
NCBI ID   WP_097115009.1    Uniprot ID   A0A286EHF4
Organism   Alysiella filiformis DSM 16848     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 583614..595686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JF568_RS02905 (JF568_02905) - 583983..584735 (-) 753 WP_097115015.1 amino acid ABC transporter ATP-binding protein -
  JF568_RS02910 (JF568_02910) - 584746..585462 (-) 717 WP_097115014.1 amino acid ABC transporter permease -
  JF568_RS02915 (JF568_02915) ttcA 585502..586446 (-) 945 WP_097115013.1 tRNA 2-thiocytidine(32) synthetase TtcA -
  JF568_RS02920 (JF568_02920) - 586612..587823 (+) 1212 WP_097115011.1 sugar transporter -
  JF568_RS02925 (JF568_02925) - 588040..588582 (-) 543 WP_097115010.1 shikimate kinase -
  JF568_RS02930 (JF568_02930) pilQ 588614..590686 (-) 2073 WP_097115009.1 type IV pilus secretin PilQ Machinery gene
  JF568_RS02935 (JF568_02935) - 590714..591247 (-) 534 WP_097115008.1 pilus assembly protein PilP -
  JF568_RS02940 (JF568_02940) - 591263..591883 (-) 621 WP_097115007.1 type 4a pilus biogenesis protein PilO -
  JF568_RS02945 (JF568_02945) - 591896..592516 (-) 621 WP_097115020.1 PilN domain-containing protein -
  JF568_RS02950 (JF568_02950) pilM 592636..593769 (-) 1134 WP_097115006.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74976.91 Da        Isoelectric Point: 9.6061

>NTDB_id=608582 JF568_RS02930 WP_097115009.1 588614..590686(-) (pilQ) [Alysiella filiformis DSM 16848]
MKPNFTKTVAALSLGMAIQAAFAGNITDINVSVLPNQQRVIKVKFDRDVVEPSGFITGSPARIALDFPNTDMQLSQNTLS
FNDSLLNQVVAAQANGRTRVLLGLTKEGEYNTQVKGNEVWVYVNEASNAVASSGTQTAGSYAAASNAPLNIDFNKGANGS
GVIKYSLDPRADAKVQVRNDRLIITIKDYPIATQDQKNLDVTDFSTPVRNIVARRLGGDTQITVTMQGAWEHRLNKRSGY
NELIIVRGDSTSSRVAQAKQQKFTGKNVSLDFQNVDVRTILQILAKESGTNIVASDNVQGQMTLVLKDVPWDQALAIVMD
ARDLGMKRNGNVINIAPRKDISAQEQSILEDQQKLENMAPLLTRNFQLKYKKVEEFREVLKITDGGSGGNNGRNSILSSR
GSVLIDPATNTLIVTDNATVIAKFEKLIDELDRLSDQVMVEARIVEAEDTVSRDLGVKFGFSRVGRTGWGNTWDNAINNR
NVVSGTGAVHTLGSNVNLPVSAATSSISLVRSISSGALGLEISAMETDNRAKTISSPRVLTQDRKAAEIKQGFQIPYQTR
DANGAYTTAFKDAVLSLKVTPNVTPDRKILLDIAINKDDVSTAFTNLDGEPAISTKQVTTQAIVEDGGTLVVGGVYQERL
GNVVSKVPVLGDLPVLGNLFKSRQRKNERNEVLFFITPRIMGAESSVMRY

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=608582 JF568_RS02930 WP_097115009.1 588614..590686(-) (pilQ) [Alysiella filiformis DSM 16848]
ATGAAACCAAATTTCACCAAAACCGTTGCTGCTTTAAGTTTGGGCATGGCAATTCAGGCAGCCTTTGCGGGCAACATTAC
CGACATCAACGTATCGGTATTGCCCAATCAACAACGTGTGATTAAAGTTAAATTTGACCGTGATGTGGTTGAGCCAAGCG
GTTTTATCACAGGTTCGCCTGCGCGTATTGCATTGGATTTCCCCAATACCGATATGCAATTATCACAAAACACATTGTCT
TTTAATGATTCTTTGCTCAATCAAGTGGTGGCGGCACAAGCAAATGGTCGCACGCGCGTGTTGTTGGGTTTGACAAAAGA
GGGCGAATACAACACCCAAGTTAAAGGCAATGAAGTGTGGGTGTATGTGAATGAAGCCAGCAATGCTGTGGCATCATCAG
GTACACAAACAGCAGGCAGCTATGCCGCCGCCAGCAATGCGCCTTTGAATATTGATTTCAATAAAGGTGCAAATGGTTCA
GGTGTGATTAAATATTCATTGGACCCACGCGCCGATGCCAAAGTGCAAGTACGCAATGACCGTTTAATCATTACCATCAA
AGATTACCCCATTGCAACACAAGACCAAAAAAATCTTGATGTAACCGATTTTTCTACGCCTGTGCGCAATATTGTGGCGC
GCCGTTTGGGTGGCGATACACAAATTACCGTTACCATGCAAGGTGCTTGGGAACACCGTTTGAACAAGAGAAGTGGCTAC
AATGAGTTGATTATTGTGCGTGGCGACAGCACTTCTTCTCGTGTGGCACAAGCCAAACAACAAAAATTTACGGGTAAAAA
TGTTTCCTTGGATTTCCAAAATGTGGATGTGCGTACCATTTTGCAAATTTTGGCAAAAGAATCAGGCACAAACATTGTGG
CAAGCGACAATGTTCAAGGGCAAATGACTTTGGTGCTGAAAGATGTGCCTTGGGATCAAGCATTGGCAATTGTGATGGAC
GCGCGTGATTTGGGCATGAAACGCAATGGCAATGTGATTAATATTGCACCACGCAAGGACATCTCTGCTCAAGAACAATC
CATTTTGGAAGACCAACAAAAATTGGAAAATATGGCGCCCTTATTGACGCGCAATTTCCAATTAAAATACAAGAAAGTGG
AAGAGTTCAGAGAAGTGTTGAAAATTACCGATGGTGGTTCGGGTGGCAATAATGGTCGCAACAGTATTTTGAGTAGTCGC
GGTAGTGTTTTGATTGACCCTGCAACCAATACTTTGATTGTAACCGACAATGCCACGGTAATTGCCAAATTTGAAAAATT
GATTGATGAGTTGGATAGATTGTCTGACCAAGTGATGGTGGAAGCGCGTATTGTGGAAGCGGAAGATACCGTGAGCCGTG
ATTTGGGCGTGAAATTTGGTTTCAGCCGCGTGGGTCGTACAGGCTGGGGCAATACTTGGGACAATGCAATTAATAACCGC
AATGTGGTTTCGGGTACAGGTGCCGTACACACATTGGGTTCTAATGTGAATTTGCCTGTATCGGCGGCAACCAGTTCCAT
TTCTTTGGTGCGTTCTATTTCGTCTGGTGCTTTGGGTTTGGAAATTTCGGCAATGGAAACGGATAACCGTGCCAAAACGA
TTTCCAGTCCACGTGTTTTAACGCAAGACCGTAAGGCTGCTGAAATCAAACAAGGTTTCCAAATTCCTTATCAAACCCGT
GATGCAAATGGTGCTTATACCACTGCATTTAAAGACGCGGTATTGAGTTTGAAAGTAACGCCAAATGTTACACCTGACCG
CAAAATTTTGTTGGACATTGCCATCAATAAAGATGATGTGAGTACGGCATTTACCAATTTAGATGGTGAACCTGCGATTT
CCACCAAACAAGTTACCACACAAGCGATTGTGGAAGATGGTGGCACTTTGGTTGTAGGTGGTGTGTATCAAGAACGATTG
GGTAATGTGGTTAGCAAGGTACCTGTTTTGGGTGATTTGCCTGTGTTGGGTAATTTGTTCAAGTCTCGTCAGAGAAAGAA
CGAGCGCAATGAAGTGCTGTTCTTCATTACCCCACGCATTATGGGTGCGGAAAGCAGTGTGATGCGCTACTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(42-117)

Bacterial type II and III secretion system protein (Secretin)

(524-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(363-436)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A286EHF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

45.862

100

0.49
  pilQ Neisseria meningitidis MC58

50

79.71

0.399
  pilQ Neisseria meningitidis M1080

50

79.71

0.399