Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   NMD35_RS03765 Genome accession   NZ_CP100973
Coordinates   780435..781094 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain ET300     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 775435..786094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD35_RS03740 (NMD35_03725) parE 775526..777418 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  NMD35_RS03745 (NMD35_03730) ygiN 777466..777780 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  NMD35_RS03750 (NMD35_03735) mdaB 777811..778392 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  NMD35_RS03755 (NMD35_03740) ygiZ 778711..779043 (+) 333 WP_000912120.1 DUF2645 family protein -
  NMD35_RS03760 (NMD35_03745) qseC 779089..780438 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  NMD35_RS03765 (NMD35_03750) ciaR 780435..781094 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  NMD35_RS03770 (NMD35_03755) ygiW 781246..781638 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  NMD35_RS03775 (NMD35_03760) ygiV 781691..782173 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  NMD35_RS03780 (NMD35_03765) parC 782719..784977 (+) 2259 WP_024249834.1 DNA topoisomerase IV subunit A -
  NMD35_RS03785 (NMD35_03770) plsC 785211..785948 (+) 738 WP_162113449.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=608001 NMD35_RS03765 WP_001221502.1 780435..781094(-) (ciaR) [Escherichia coli strain ET300]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=608001 NMD35_RS03765 WP_001221502.1 780435..781094(-) (ciaR) [Escherichia coli strain ET300]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365