Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   K9N68_RS27390 Genome accession   NZ_CP083582
Coordinates   5853515..5855512 (+) Length   665 a.a.
NCBI ID   WP_224341402.1    Uniprot ID   -
Organism   Kovacikia minuta CCNUW1 strain CCNU0001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5848515..5860512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9N68_RS27375 (K9N68_27375) dnaJ 5848570..5849697 (-) 1128 WP_224341400.1 molecular chaperone DnaJ -
  K9N68_RS27380 (K9N68_27380) dnaK 5849822..5851775 (-) 1954 Protein_5621 molecular chaperone DnaK -
  K9N68_RS27385 (K9N68_27385) grpE 5851971..5852714 (-) 744 WP_224341401.1 nucleotide exchange factor GrpE -
  K9N68_RS27390 (K9N68_27390) pilB/pilB1 5853515..5855512 (+) 1998 WP_224341402.1 GspE/PulE family protein Machinery gene
  K9N68_RS27395 (K9N68_27395) pilT 5855701..5856816 (+) 1116 WP_224341403.1 type IV pilus twitching motility protein PilT Machinery gene
  K9N68_RS27400 (K9N68_27400) - 5856978..5858201 (+) 1224 WP_224341404.1 type II secretion system F family protein -
  K9N68_RS27405 (K9N68_27405) - 5858328..5858930 (+) 603 WP_224341405.1 hypothetical protein -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 74609.36 Da        Isoelectric Point: 5.0076

>NTDB_id=606347 K9N68_RS27390 WP_224341402.1 5853515..5855512(+) (pilB/pilB1) [Kovacikia minuta CCNUW1 strain CCNU0001]
MTNSSSQRRALVVQNNFSPFGNKLIQSGYVNNEQMQQALIESRKSGRALTDVLESITGQQLPPDLLRQYKKQQLFELKIL
YGVESLDPEISQIGTTQVSELIENLIPVDLCRTHKLVPLSRSDGQPPFLLVAMVDPDDLRAQDDLNRILRPQGLALQRLV
ITLEDYRRLINEYLDDKEKRDKQIEAQSKVDVQREMEGLDYLDLQDAPDDEDANLDVADAEAAPVIALVNKIMIKALQEG
VSDIHIEPQEEVLRVRFRKDGVLRQAFEPFPKKIIPAVTARFKIISQLDIAERRAPQDGRIRRIYDGRKIDFRVNTLPSR
YGEKVVLRILDNSATQLGLDKLIADPESLQIVQEMVRRPFGLILVTGPTGSGKTTTLYSALAERNDPGVNISTAEDPIEY
TLPGLTQVQVIREKGLDFAAILRAFLRQDPDVILVGETRDRETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVEP
FMVSGALLGVVAQRLVRRVCDECRIPYTPSREELARFGLSASRDGELTLYKANTLQPNEIQEARARSQLCPKCSGIGYKG
RCGVYEVMRVTERLQNMINQGAPTEAIKEVAVEEGMQTLLAYSLNLVRQGFTTLEEVERVTFTDTGLEAELRAKRKTSLS
CRVCSAELKQEWLDCPYCLTPRFQD

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=606347 K9N68_RS27390 WP_224341402.1 5853515..5855512(+) (pilB/pilB1) [Kovacikia minuta CCNUW1 strain CCNU0001]
ATGACTAACTCCTCCTCTCAGCGTCGTGCGCTTGTCGTCCAAAATAATTTTTCTCCGTTCGGAAATAAGCTAATTCAGTC
TGGGTACGTCAACAATGAACAGATGCAGCAGGCGCTGATTGAGAGCCGCAAGTCTGGTCGAGCACTGACAGACGTGCTGG
AGTCAATTACAGGGCAACAACTTCCCCCCGATCTGTTACGTCAATACAAGAAGCAGCAACTCTTCGAACTCAAGATTCTC
TACGGAGTCGAATCGCTTGACCCTGAAATTAGTCAGATTGGAACGACTCAGGTCAGCGAACTCATCGAAAACTTGATTCC
GGTCGATCTCTGCCGCACCCACAAGCTGGTTCCCCTTTCTAGAAGTGATGGTCAGCCCCCATTTCTGCTGGTTGCGATGG
TCGATCCAGACGATCTGCGCGCCCAGGACGATTTGAACCGGATTTTGCGTCCTCAGGGGCTGGCGTTGCAACGACTCGTG
ATTACCCTGGAAGACTATCGACGGCTAATTAACGAATATCTGGACGACAAGGAAAAACGCGACAAGCAGATTGAGGCACA
ATCTAAGGTCGATGTTCAGAGGGAAATGGAAGGGCTGGACTACCTGGATTTGCAGGATGCGCCCGATGATGAAGACGCGA
ATCTGGATGTTGCCGATGCAGAAGCGGCTCCAGTGATTGCCCTGGTGAACAAAATCATGATTAAAGCCCTTCAGGAAGGG
GTTTCTGATATTCACATTGAGCCTCAAGAAGAAGTTTTACGGGTTCGCTTCCGTAAAGATGGCGTGTTGCGTCAGGCATT
TGAGCCTTTCCCTAAGAAAATTATTCCAGCTGTCACTGCCCGCTTCAAAATCATTTCTCAGCTAGATATTGCTGAACGAC
GGGCACCTCAGGACGGGCGGATTCGCCGGATTTATGACGGGCGCAAGATTGACTTCCGGGTTAATACCCTGCCCAGCCGT
TACGGCGAAAAGGTCGTTCTACGGATTTTGGATAATTCGGCAACACAATTGGGGCTAGACAAGCTGATTGCAGATCCAGA
ATCGTTGCAGATTGTACAAGAAATGGTTCGTCGTCCCTTTGGACTGATTTTGGTGACTGGACCAACCGGGTCTGGTAAAA
CAACCACCCTGTATTCTGCCCTGGCTGAACGGAACGATCCGGGTGTCAATATCAGTACGGCAGAAGACCCAATCGAATAT
ACCCTCCCTGGTCTAACTCAGGTGCAGGTGATTCGGGAAAAGGGTTTAGACTTCGCTGCCATTCTAAGAGCCTTTCTGCG
TCAAGATCCGGATGTAATTCTGGTGGGTGAAACCCGCGATCGTGAAACGGCAAAGACCGCGATCGAGGCAGCCCTAACTG
GACACCTGGTATTAACCACCCTCCACACAAACGACGCCGCAGGGGCGATTGCCCGCCTGGATGAAATGGGGGTTGAACCC
TTCATGGTATCCGGTGCCTTGCTGGGAGTTGTTGCTCAGCGGCTAGTCCGGCGCGTGTGCGACGAATGCCGTATTCCCTA
CACTCCCTCGCGGGAAGAACTGGCAAGATTTGGCCTATCTGCATCCCGTGACGGTGAGTTGACTCTGTACAAAGCAAACA
CCCTACAGCCCAACGAAATTCAGGAAGCCAGAGCTAGAAGCCAACTCTGCCCAAAATGTAGTGGTATCGGCTACAAAGGA
CGTTGTGGGGTTTATGAAGTGATGCGCGTCACAGAACGGCTCCAGAACATGATTAACCAGGGTGCGCCCACTGAAGCTAT
TAAAGAAGTGGCTGTGGAAGAAGGGATGCAAACCTTATTGGCATACAGCCTCAACCTTGTGCGTCAAGGTTTCACTACCC
TGGAAGAGGTCGAACGGGTAACCTTTACCGATACCGGCCTGGAAGCTGAACTGAGAGCCAAGCGAAAGACCTCCCTCAGT
TGTCGTGTTTGTAGCGCCGAGCTGAAGCAAGAATGGCTGGATTGTCCCTACTGCCTCACCCCTCGCTTCCAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(70-178)

Type II/IV secretion system protein (T2SSE)

(231-498)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

67.922

99.85

0.678
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.484

93.233

0.377
  pilF Thermus thermophilus HB27

39.61

92.632

0.367