Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NI383_RS20440 Genome accession   NZ_CP099955
Coordinates   2671707..2672159 (+) Length   150 a.a.
NCBI ID   WP_029846089.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain Isc4A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2666707..2677159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI383_RS20425 (NI383_20580) pdhR 2668740..2669507 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NI383_RS20430 (NI383_20585) ampD 2669913..2670464 (-) 552 WP_140284224.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI383_RS20435 (NI383_20590) nadC 2670557..2671444 (+) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI383_RS20440 (NI383_20595) pilA 2671707..2672159 (+) 453 WP_029846089.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NI383_RS20445 (NI383_20600) pilB 2672161..2673846 (+) 1686 WP_315868304.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI383_RS20450 (NI383_20605) pilC 2673870..2675093 (+) 1224 WP_315868305.1 type II secretion system F family protein Machinery gene
  NI383_RS20455 (NI383_20610) pilD 2675158..2676027 (+) 870 WP_140154970.1 A24 family peptidase Machinery gene
  NI383_RS20460 (NI383_20615) coaE 2676028..2676642 (+) 615 WP_005480887.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15708.01 Da        Isoelectric Point: 7.8461

>NTDB_id=603786 NI383_RS20440 WP_029846089.1 2671707..2672159(+) (pilA) [Vibrio parahaemolyticus strain Isc4A]
MKHSKQKKQQGFTLIELMIVVGIIGIISALAVPAYKSYVLKTEANTAVGVPRALLANVDLFVQEKGKYPNSTQTADLAAI
GAAIDMSAMGTLAITPDADGSEYGDIEFTIGSNASLSGKKVTFARSTNGWKCTHDTGQDLKGCATTPATP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=603786 NI383_RS20440 WP_029846089.1 2671707..2672159(+) (pilA) [Vibrio parahaemolyticus strain Isc4A]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTGGTTGGGATTATTGGGATTAT
AAGTGCATTAGCTGTACCAGCTTATAAAAGCTATGTACTAAAAACCGAAGCTAATACTGCTGTGGGCGTGCCAAGAGCCT
TGTTAGCAAACGTAGACCTCTTCGTCCAAGAAAAAGGTAAATACCCTAATTCTACTCAAACTGCCGATCTTGCAGCTATC
GGAGCTGCTATAGATATGAGTGCGATGGGAACTCTTGCTATCACTCCTGACGCAGACGGTTCTGAGTACGGGGACATTGA
GTTTACCATTGGCTCTAATGCTTCATTGAGTGGCAAAAAGGTTACGTTTGCTCGCTCAACTAACGGTTGGAAGTGTACGC
ATGATACAGGTCAAGACCTTAAAGGCTGTGCTACCACTCCAGCAACTCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(56-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

37.838

98.667

0.373
  pilA Vibrio parahaemolyticus RIMD 2210633

42.105

88.667

0.373