Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   NI388_RS03015 Genome accession   NZ_CP099919
Coordinates   618587..619045 (-) Length   152 a.a.
NCBI ID   WP_021486793.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain Isc14B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 613587..624045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI388_RS02995 (NI388_02995) coaE 614106..614720 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  NI388_RS03000 (NI388_03000) pilD 614721..615590 (-) 870 WP_025533026.1 A24 family peptidase Machinery gene
  NI388_RS03005 (NI388_03005) pilC 615655..616878 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  NI388_RS03010 (NI388_03010) pilB 616902..618587 (-) 1686 WP_029855968.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI388_RS03015 (NI388_03015) pilE 618587..619045 (-) 459 WP_021486793.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NI388_RS03020 (NI388_03020) nadC 619309..620196 (-) 888 WP_025638275.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI388_RS03025 (NI388_03025) ampD 620289..620840 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI388_RS03030 (NI388_03030) pdhR 621246..622013 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15442.56 Da        Isoelectric Point: 5.7425

>NTDB_id=603333 NI388_RS03015 WP_021486793.1 618587..619045(-) (pilE) [Vibrio parahaemolyticus strain Isc14B]
MKHSKQKKQQGFTLIELMIVVAIIGILAAFAVPAYSDYTQRTRVAGAAAGISGFKTAIAMCAQERGQLTGCSNGANDIPA
AIAANNAGATIAYVDDLTVTDGVIAMTTTGVNDSGTELTLTLTPNIGNGVVQWTLAGTGCTTAGRSIDCSGN

Nucleotide


Download         Length: 459 bp        

>NTDB_id=603333 NI388_RS03015 WP_021486793.1 618587..619045(-) (pilE) [Vibrio parahaemolyticus strain Isc14B]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACGCTAATTGAATTGATGATTGTGGTGGCGATTATCGGTATTTT
GGCTGCATTTGCCGTGCCTGCCTACTCAGACTACACACAACGAACCCGTGTGGCCGGTGCAGCTGCAGGGATCAGTGGTT
TTAAAACAGCCATTGCCATGTGTGCTCAGGAACGTGGCCAGCTTACCGGTTGTAGCAACGGCGCAAATGATATCCCGGCA
GCAATTGCCGCGAATAACGCCGGTGCGACCATTGCTTATGTGGACGACTTAACGGTTACCGATGGCGTTATCGCCATGAC
CACGACCGGAGTTAATGACTCAGGTACCGAGCTAACACTGACGTTAACACCGAATATTGGCAATGGCGTTGTTCAGTGGA
CACTTGCTGGTACTGGCTGTACAACCGCTGGACGCAGCATTGATTGTTCTGGTAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

39.726

96.053

0.382
  pilA2 Legionella pneumophila str. Paris

38.462

94.079

0.362
  pilE Neisseria gonorrhoeae strain FA1090

38.194

94.737

0.362