Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   K6752_RS01595 Genome accession   NZ_CP082315
Coordinates   339567..340160 (+) Length   197 a.a.
NCBI ID   WP_005381322.1    Uniprot ID   A0AAW9BKV9
Organism   Vibrio alginolyticus strain ZLV3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 334567..345160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6752_RS01580 (K6752_01580) - 335293..337854 (-) 2562 WP_005381325.1 penicillin-binding protein 1A -
  K6752_RS01585 (K6752_01585) pilM 337999..339015 (+) 1017 WP_005381324.1 type IV pilus biogenesis protein PilM Machinery gene
  K6752_RS01590 (K6752_01590) pilN 338999..339574 (+) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  K6752_RS01595 (K6752_01595) pilO 339567..340160 (+) 594 WP_005381322.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6752_RS01600 (K6752_01600) pilP 340150..340665 (+) 516 WP_005381321.1 pilus assembly protein PilP Machinery gene
  K6752_RS01605 (K6752_01605) pilQ 340691..342436 (+) 1746 WP_031779065.1 type IV pilus secretin PilQ Machinery gene
  K6752_RS01610 (K6752_01610) aroK 342624..343142 (+) 519 WP_005381319.1 shikimate kinase AroK -
  K6752_RS01615 (K6752_01615) aroB 343175..344275 (+) 1101 WP_005381318.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23015.53 Da        Isoelectric Point: 5.0885

>NTDB_id=601315 K6752_RS01595 WP_005381322.1 339567..340160(+) (pilO) [Vibrio alginolyticus strain ZLV3]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMVIQGLGYWFYLKPKDEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=601315 K6752_RS01595 WP_005381322.1 339567..340160(+) (pilO) [Vibrio alginolyticus strain ZLV3]
GTGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGACGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACCCGTTACGAGTTATTACTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
ACTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAAAGCGAAACCCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.368

96.447

0.843
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685