Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K6752_RS01585 Genome accession   NZ_CP082315
Coordinates   337999..339015 (+) Length   338 a.a.
NCBI ID   WP_005381324.1    Uniprot ID   A0AAW9BNQ7
Organism   Vibrio alginolyticus strain ZLV3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 332999..344015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6752_RS01570 (K6752_01570) - 333430..334158 (-) 729 WP_005382781.1 redoxin family protein -
  K6752_RS01575 (K6752_01575) oxyR 334328..335239 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  K6752_RS01580 (K6752_01580) - 335293..337854 (-) 2562 WP_005381325.1 penicillin-binding protein 1A -
  K6752_RS01585 (K6752_01585) pilM 337999..339015 (+) 1017 WP_005381324.1 type IV pilus biogenesis protein PilM Machinery gene
  K6752_RS01590 (K6752_01590) pilN 338999..339574 (+) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  K6752_RS01595 (K6752_01595) pilO 339567..340160 (+) 594 WP_005381322.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6752_RS01600 (K6752_01600) pilP 340150..340665 (+) 516 WP_005381321.1 pilus assembly protein PilP Machinery gene
  K6752_RS01605 (K6752_01605) pilQ 340691..342436 (+) 1746 WP_031779065.1 type IV pilus secretin PilQ Machinery gene
  K6752_RS01610 (K6752_01610) aroK 342624..343142 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37581.92 Da        Isoelectric Point: 7.2224

>NTDB_id=601313 K6752_RS01585 WP_005381324.1 337999..339015(+) (pilM) [Vibrio alginolyticus strain ZLV3]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=601313 K6752_RS01585 WP_005381324.1 337999..339015(+) (pilM) [Vibrio alginolyticus strain ZLV3]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGTGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACTTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTTTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503