Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   K6745_RS13910 Genome accession   NZ_CP082303
Coordinates   2992788..2993363 (-) Length   191 a.a.
NCBI ID   WP_017821732.1    Uniprot ID   A0A2I3CBW9
Organism   Vibrio alginolyticus strain FJV2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2987788..2998363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6745_RS13885 (K6745_13800) aroB 2988087..2989187 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  K6745_RS13890 (K6745_13805) aroK 2989220..2989738 (-) 519 WP_005381319.1 shikimate kinase AroK -
  K6745_RS13895 (K6745_13810) pilQ 2989926..2991671 (-) 1746 WP_042523674.1 type IV pilus secretin PilQ Machinery gene
  K6745_RS13900 (K6745_13815) pilP 2991697..2992212 (-) 516 WP_205461596.1 pilus assembly protein PilP Machinery gene
  K6745_RS13905 (K6745_13820) pilO 2992202..2992795 (-) 594 WP_213916785.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6745_RS13910 (K6745_13825) pilN 2992788..2993363 (-) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  K6745_RS13915 (K6745_13830) pilM 2993347..2994363 (-) 1017 WP_213916784.1 type IV pilus biogenesis protein PilM Machinery gene
  K6745_RS13920 (K6745_13835) - 2994508..2997069 (+) 2562 WP_213916783.1 penicillin-binding protein 1A -
  K6745_RS13925 (K6745_13840) oxyR 2997123..2998034 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22349.86 Da        Isoelectric Point: 7.4658

>NTDB_id=601156 K6745_RS13910 WP_017821732.1 2992788..2993363(-) (pilN) [Vibrio alginolyticus strain FJV2]
MLYRINLLPWRESQREEHRRRFISLVALGMLVAVGIQWGIGKFFEYQQDQQQERLDYLTHYIAELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPKLATMLDNMERSAKLNDVEMHSIV
HGKARFGKEFQTFKVSFMFKVTEKKLEAQRG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=601156 K6745_RS13910 WP_017821732.1 2992788..2993363(-) (pilN) [Vibrio alginolyticus strain FJV2]
GTGCTTTATCGCATTAATTTACTTCCTTGGCGAGAGAGTCAAAGAGAAGAGCACCGACGTCGTTTCATCAGTTTGGTCGC
ATTAGGCATGTTGGTCGCTGTTGGCATCCAGTGGGGTATTGGAAAGTTTTTTGAGTACCAACAAGATCAACAACAGGAGC
GGCTCGATTACCTCACTCACTACATTGCCGAACTCGACCAGCGTATTGAAGCAATGAAAATTGCAGAGCAAGAGCATAGC
AAAATCCTCGAACGCCTAAAGGTCGTTGAAGGTTTACAAAACGGACGAAACAAAACGACCGAGTTCATGAACTTAATGCC
TGCCGTTATCCCTGAAGGTGTATATGTCGACAAGATCAAAATGAATGATCTGGAAATTGAGATCTCTGGGATCTCAGATA
GCACTCCAAAACTTGCCACGATGCTGGACAACATGGAGCGCTCGGCCAAGTTGAACGACGTAGAAATGCATTCCATCGTT
CACGGCAAAGCCCGTTTTGGGAAAGAGTTTCAAACATTCAAAGTGTCTTTCATGTTCAAAGTAACGGAGAAGAAATTGGA
GGCTCAACGTGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3CBW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.199

100

0.822
  pilN Vibrio cholerae strain A1552

63.277

92.67

0.586