Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K6745_RS13915 Genome accession   NZ_CP082303
Coordinates   2993347..2994363 (-) Length   338 a.a.
NCBI ID   WP_213916784.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FJV2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2988347..2999363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6745_RS13890 (K6745_13805) aroK 2989220..2989738 (-) 519 WP_005381319.1 shikimate kinase AroK -
  K6745_RS13895 (K6745_13810) pilQ 2989926..2991671 (-) 1746 WP_042523674.1 type IV pilus secretin PilQ Machinery gene
  K6745_RS13900 (K6745_13815) pilP 2991697..2992212 (-) 516 WP_205461596.1 pilus assembly protein PilP Machinery gene
  K6745_RS13905 (K6745_13820) pilO 2992202..2992795 (-) 594 WP_213916785.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6745_RS13910 (K6745_13825) pilN 2992788..2993363 (-) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  K6745_RS13915 (K6745_13830) pilM 2993347..2994363 (-) 1017 WP_213916784.1 type IV pilus biogenesis protein PilM Machinery gene
  K6745_RS13920 (K6745_13835) - 2994508..2997069 (+) 2562 WP_213916783.1 penicillin-binding protein 1A -
  K6745_RS13925 (K6745_13840) oxyR 2997123..2998034 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  K6745_RS13930 (K6745_13845) - 2998204..2998932 (+) 729 WP_054866559.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37638.09 Da        Isoelectric Point: 7.2090

>NTDB_id=601157 K6745_RS13915 WP_213916784.1 2993347..2994363(-) (pilM) [Vibrio alginolyticus strain FJV2]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGIRGVNCASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=601157 K6745_RS13915 WP_213916784.1 2993347..2994363(-) (pilM) [Vibrio alginolyticus strain FJV2]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCATACGCGGAGTAAACTGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTGGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.84

100

0.828
  pilM Vibrio cholerae strain A1552

50.149

99.112

0.497