Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   M5C73_RS05605 Genome accession   NZ_CP098790
Coordinates   1142388..1143710 (-) Length   440 a.a.
NCBI ID   WP_252160324.1    Uniprot ID   -
Organism   Lactococcus lactis strain WiKim0124     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1137388..1148710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5C73_RS05575 (M5C73_05575) - 1138531..1139499 (+) 969 WP_003130649.1 PhoH family protein -
  M5C73_RS05580 (M5C73_05580) - 1139557..1140036 (+) 480 WP_058223061.1 NUDIX hydrolase -
  M5C73_RS05585 (M5C73_05585) ybeY 1140188..1140676 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  M5C73_RS05590 (M5C73_05590) - 1140660..1141115 (+) 456 WP_003130653.1 diacylglycerol kinase -
  M5C73_RS05595 (M5C73_05595) - 1141129..1141707 (+) 579 WP_023189343.1 nucleotidyltransferase family protein -
  M5C73_RS05600 (M5C73_05600) comFC 1141741..1142145 (-) 405 WP_021722514.1 ComF family protein Machinery gene
  M5C73_RS05605 (M5C73_05605) comFA 1142388..1143710 (-) 1323 WP_252160324.1 DEAD/DEAH box helicase Machinery gene
  M5C73_RS05610 (M5C73_05610) - 1143767..1144396 (+) 630 WP_003130660.1 YigZ family protein -
  M5C73_RS05615 (M5C73_05615) - 1144530..1145027 (+) 498 WP_003130662.1 VanZ family protein -
  M5C73_RS05620 (M5C73_05620) - 1145075..1146094 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  M5C73_RS05625 (M5C73_05625) - 1146210..1146956 (+) 747 WP_252160325.1 bifunctional metallophosphatase/5'-nucleotidase -
  M5C73_RS05630 (M5C73_05630) glyQ 1147241..1148194 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50293.90 Da        Isoelectric Point: 10.1843

>NTDB_id=601148 M5C73_RS05605 WP_252160324.1 1142388..1143710(-) (comFA) [Lactococcus lactis strain WiKim0124]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKKSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDDDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNKMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIES
GQEFAKQLQLKFPKEKITFVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=601148 M5C73_RS05605 WP_252160324.1 1142388..1143710(-) (comFA) [Lactococcus lactis strain WiKim0124]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAAATCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGACGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATAAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATCT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTTCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

52.261

90.455

0.473
  comFA/cflA Streptococcus pneumoniae Rx1

52.261

90.455

0.473
  comFA/cflA Streptococcus pneumoniae D39

52.261

90.455

0.473
  comFA/cflA Streptococcus pneumoniae R6

52.261

90.455

0.473
  comFA/cflA Streptococcus pneumoniae TIGR4

52.261

90.455

0.473
  comFA/cflA Streptococcus mitis NCTC 12261

51.508

90.455

0.466
  comFA Bacillus subtilis subsp. subtilis str. 168

36.807

100

0.377