Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   K4A81_RS18215 Genome accession   NZ_CP082262
Coordinates   3662273..3663658 (-) Length   461 a.a.
NCBI ID   WP_029972824.1    Uniprot ID   A0AAP4DHF4
Organism   Bacillus velezensis strain BIM B-454D     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3657273..3668658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4A81_RS18180 (K4A81_18180) flgL 3657448..3658365 (-) 918 WP_007407477.1 flagellar hook-associated protein FlgL -
  K4A81_RS18185 (K4A81_18185) flgK 3658377..3659894 (-) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  K4A81_RS18190 (K4A81_18190) - 3659910..3660392 (-) 483 WP_003151394.1 flagellar protein FlgN -
  K4A81_RS18195 (K4A81_18195) flgM 3660407..3660673 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  K4A81_RS18200 (K4A81_18200) - 3660743..3661162 (-) 420 WP_014419009.1 TIGR03826 family flagellar region protein -
  K4A81_RS18205 (K4A81_18205) comFC 3661236..3661925 (-) 690 WP_199449054.1 ComF family protein Machinery gene
  K4A81_RS18210 (K4A81_18210) - 3661931..3662215 (-) 285 WP_003151389.1 late competence development ComFB family protein -
  K4A81_RS18215 (K4A81_18215) comFA 3662273..3663658 (-) 1386 WP_029972824.1 DEAD/DEAH box helicase Machinery gene
  K4A81_RS18220 (K4A81_18220) - 3663766..3664614 (-) 849 WP_003151387.1 DegV family protein -
  K4A81_RS18225 (K4A81_18225) degU 3664712..3665401 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  K4A81_RS18230 (K4A81_18230) degS 3665478..3666641 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  K4A81_RS18235 (K4A81_18235) - 3666864..3667511 (+) 648 WP_007407485.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52662.09 Da        Isoelectric Point: 10.2802

>NTDB_id=600585 K4A81_RS18215 WP_029972824.1 3662273..3663658(-) (comFA) [Bacillus velezensis strain BIM B-454D]
MSEGDSEFSEVRDFFYGRRLLRSEIPFSDQRIKRLTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLNKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQLQLPVFLFVPSVHVLKKTTDYFRKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=600585 K4A81_RS18215 WP_029972824.1 3662273..3663658(-) (comFA) [Bacillus velezensis strain BIM B-454D]
ATGTCCGAAGGTGATTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCGTTTATTGAGATCTGAGATACCGTT
TTCAGACCAGCGCATCAAGCGTTTAACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTTTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTTTGTGTCGCAACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAATAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCGACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTCCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCGGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(330-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.174

99.783

0.62