Detailed information NTG0600508

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NDQ70_RS10300
• Genome accession: NZ_CP098534
• Coordinates: 1941374..1942600 (-)
• Length: 408 a.a.
• NCBI ID: WP_003697786.1
• Uniprot ID: B4RPS5
• Organism: Neisseria gonorrhoeae strain 10525
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1936374..1947600

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NDQ70_RS10290 (NDQ70_10095)	-	1938339..1938623 (+)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
NDQ70_RS10295 (NDQ70_10100)	yccS	1939107..1941257 (-)	2151	WP_003697785.1	YccS family putative transporter	-
NDQ70_RS10300 (NDQ70_10105)	pilU	1941374..1942600 (-)	1227	WP_003697786.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NDQ70_RS10305 (NDQ70_10110)	pilT	1942764..1943807 (-)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
NDQ70_RS10310 (NDQ70_10115)	-	1943927..1944613 (+)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
NDQ70_RS10315 (NDQ70_10120)	-	1944613..1945020 (+)	408	WP_003690202.1	hypothetical protein	-
NDQ70_RS10320 (NDQ70_10125)	proC	1945069..1945860 (+)	792	WP_003694504.1	pyrroline-5-carboxylate reductase	-
NDQ70_RS10325 (NDQ70_10130)	dksA	1946009..1946425 (+)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
NDQ70_RS10330 (NDQ70_12040)	-	1946440..1946712 (+)	273	WP_162855536.1	hypothetical protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45668.30 Da
• Isoelectric Point: 7.2909

>NTDB_id=600508 NDQ70_RS10300 WP_003697786.1 1941374..1942600(-) (pilU) [Neisseria gonorrhoeae strain 10525]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=600508 NDQ70_RS10300 WP_003697786.1 1941374..1942600(-) (pilU) [Neisseria gonorrhoeae strain 10525]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: B4RPS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387