Detailed information NTG0599474

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ABWED_RS01640
• Genome accession: NZ_CP082143
• Coordinates: 368859..369917 (+)
• Length: 352 a.a.
• NCBI ID: WP_004644949.1
• Uniprot ID: N9Q4M1
• Organism: Acinetobacter lwoffii strain ED23-35
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 363859..374917

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABWED_RS01625 (ABWED_0336)	mraY	364141..365259 (+)	1119	WP_005265518.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
ABWED_RS01630 (ABWED_0337)	-	365314..366117 (+)	804	WP_374956143.1	putative RNA methyltransferase	-
ABWED_RS01635 (ABWED_0338)	ponA	366168..368693 (-)	2526	WP_374956144.1	penicillin-binding protein PBP1a	-
ABWED_RS01640 (ABWED_0339)	pilM	368859..369917 (+)	1059	WP_004644949.1	pilus assembly protein PilM	Machinery gene
ABWED_RS01645 (ABWED_0340)	comN	369917..370555 (+)	639	WP_004281141.1	PilN domain-containing protein	Machinery gene
ABWED_RS01650 (ABWED_0341)	comO	370552..371262 (+)	711	WP_374956145.1	type 4a pilus biogenesis protein PilO	Machinery gene
ABWED_RS01655 (ABWED_0342)	pilP	371262..371789 (+)	528	WP_005248368.1	pilus assembly protein PilP	Machinery gene
ABWED_RS01660 (ABWED_0343)	pilQ	371807..373975 (+)	2169	WP_374956146.1	type IV pilus secretin PilQ	Machinery gene
ABWED_RS01665 (ABWED_0344)	aroK	374012..374548 (+)	537	WP_005101380.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39037.53 Da
• Isoelectric Point: 4.5358

>NTDB_id=599474 ABWED_RS01640 WP_004644949.1 368859..369917(+) (pilM) [Acinetobacter lwoffii strain ED23-35]
MRRLYRKPNKGLIGVDISSTSVKVLELSVKSGRYWVESYALIPLSENSVVEKNILNPEAVADALGRAINLGNTQSNQAAF
AIPTSMVITKIIEMDADMSDDEREVQIREDAEQYIPFPLDEASLDFEVLPDRLSNPNRVNVLLVATRIENVEARSEVLEI
SSLTPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=599474 ABWED_RS01640 WP_004644949.1 368859..369917(+) (pilM) [Acinetobacter lwoffii strain ED23-35]
GTGCGCAGGTTATATCGTAAACCAAATAAGGGGTTAATAGGAGTCGATATTAGTTCGACTTCTGTTAAGGTTTTAGAACT
TTCTGTAAAAAGTGGCCGTTACTGGGTAGAGAGCTATGCTTTGATCCCATTATCGGAAAACAGTGTTGTTGAAAAAAACA
TTTTAAACCCAGAAGCAGTCGCTGATGCCCTTGGGCGTGCAATTAATTTGGGTAATACCCAGTCGAATCAGGCCGCTTTT
GCGATTCCAACTTCAATGGTCATCACCAAAATCATTGAGATGGATGCAGACATGTCAGATGATGAACGTGAAGTTCAGAT
TCGTGAAGATGCAGAACAATACATTCCTTTCCCACTCGATGAAGCAAGTCTGGATTTTGAAGTTCTGCCAGACCGCCTTT
CCAATCCGAACCGGGTCAATGTACTGCTGGTCGCTACCCGAATTGAAAATGTCGAAGCACGTTCTGAAGTTTTAGAGATT
AGCAGTCTAACGCCTAAGATTGCAGATGTAGAAAGTTTTGCGCTGGAAAATGCCTTTAAGGTATTTTCGGATACTTTACC
GATGGGGGTCAATACGGTTGGTATTTTAGACATTGGTCATAGTATGACGACCTTGTCAGTGATGCAGAACAACAAGATTA
TTTATACTCGTGAGCAGGTGTTTGGCGGTAAGCAATTAACTCAGGAAATTCAGAATCGTTATGGATTATCCTTTGAAGAA
GCTGGACGTGCTAAAAAAACCCGTGCCTTGCCTGATGATTATGATATTGAAGTCCTAGAACCCTTTTTGGATGCCGTGGT
ACAACAGGCAGCCCGTTCATTACAATTCTTCTTTTCATCTTCTCAGTTTAATGAGATTGACCATATTTTACTGGCCGGCG
GGAATGCCAATATTCCAGGTCTGGCAAAATTATTACAACAAAAACTCGGTTACCGTGTCACGATCGCGAATCCATTTTTG
CAAATGGGCTTTTCTCCTCAAATTGATATTAAAAAAATTGAAAATGACGCGTCATCACTCATGGTTGCATGCGGTTTGGC
ATTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9Q4M1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	79.261	100	0.793
pilM	Legionella pneumophila strain ERS1305867	39.266	100	0.395