Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K5O18_RS00185 Genome accession   NZ_CP081939
Coordinates   40187..40729 (+) Length   180 a.a.
NCBI ID   WP_110479719.1    Uniprot ID   A0AAE5TGK8
Organism   Avibacterium paragallinarum strain p4chr1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 35187..45729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5O18_RS00160 (K5O18_00160) - 35668..36597 (+) 930 WP_046097867.1 phosphatidate cytidylyltransferase -
  K5O18_RS00165 (K5O18_00165) mog 36616..37194 (+) 579 WP_110479722.1 molybdopterin adenylyltransferase -
  K5O18_RS00170 (K5O18_00170) glnB 37223..37561 (+) 339 WP_017805299.1 nitrogen regulatory protein P-II -
  K5O18_RS00175 (K5O18_00175) - 37564..38616 (+) 1053 WP_110479721.1 AI-2E family transporter -
  K5O18_RS00180 (K5O18_00180) - 38984..40153 (+) 1170 WP_110479720.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K5O18_RS00185 (K5O18_00185) pilF/pilF2 40187..40729 (+) 543 WP_110479719.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K5O18_RS00190 (K5O18_00190) dprA 40874..41974 (+) 1101 WP_110479718.1 DNA-processing protein DprA Machinery gene
  K5O18_RS00195 (K5O18_00195) rsmD 41971..42579 (-) 609 WP_110479717.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  K5O18_RS00200 (K5O18_00200) ftsY 42735..44078 (+) 1344 WP_222593712.1 signal recognition particle-docking protein FtsY -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20727.78 Da        Isoelectric Point: 8.0752

>NTDB_id=598518 K5O18_RS00185 WP_110479719.1 40187..40729(+) (pilF/pilF2) [Avibacterium paragallinarum strain p4chr1]
MKIYQCILSILFSLFLVACVSTPLNKQDFDKQQAAKARVELGIGYLEQNELGLAKQNLDKALAYAPNYYLVHSALAYFYQ
LQGENIRAENAYLKALKLDDKQGDVFNNYAVFLCKQGQFEQAYLRFEQALATPNYYQQADTYENIVLCALAEKNAIRYEK
YKGLLEKLDPKRNQKTNVIH

Nucleotide


Download         Length: 543 bp        

>NTDB_id=598518 K5O18_RS00185 WP_110479719.1 40187..40729(+) (pilF/pilF2) [Avibacterium paragallinarum strain p4chr1]
ATGAAAATTTATCAATGTATCTTAAGCATTCTTTTTTCTCTCTTTCTCGTAGCCTGTGTTTCCACTCCCTTAAATAAGCA
AGACTTTGATAAGCAGCAAGCCGCAAAGGCGAGAGTGGAACTTGGCATTGGCTATCTTGAGCAAAATGAACTTGGTCTGG
CGAAGCAAAATTTAGACAAAGCCTTAGCTTATGCCCCGAATTATTATTTAGTGCATTCAGCGTTGGCATATTTTTATCAG
CTTCAAGGGGAGAATATTCGTGCGGAAAATGCGTATTTGAAAGCCCTAAAACTCGACGATAAACAAGGCGATGTATTTAA
TAATTATGCCGTCTTTTTGTGCAAACAAGGACAATTCGAACAGGCTTATCTACGATTTGAACAAGCTCTCGCCACACCTA
ATTATTATCAGCAAGCGGATACTTACGAGAATATTGTACTTTGTGCGTTAGCAGAGAAAAATGCAATACGTTATGAAAAA
TATAAAGGCTTGCTGGAGAAACTAGATCCTAAAAGAAATCAAAAGACAAATGTTATTCATTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

49.153

98.333

0.483
  pilF Glaesserella parasuis strain SC1401

43.407

100

0.439