Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J451_RS13090 Genome accession   NC_021739
Coordinates   2559607..2560086 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica D174     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2554607..2565086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J451_RS13060 (J451_13140) - 2555188..2555952 (-) 765 WP_006248395.1 TSUP family transporter -
  J451_RS13065 (J451_14015) proB 2555952..2557036 (-) 1085 Protein_2580 glutamate 5-kinase -
  J451_RS13075 (J451_13145) folA 2557148..2557642 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  J451_RS13080 (J451_13150) - 2557762..2557992 (+) 231 WP_006248398.1 YgjV family protein -
  J451_RS13085 (J451_13155) radA 2558047..2559426 (-) 1380 WP_020824406.1 DNA repair protein RadA -
  J451_RS13090 (J451_13160) pilA 2559607..2560086 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  J451_RS13095 (J451_13165) - 2560107..2560550 (+) 444 Protein_2585 pilus assembly protein PilB -
  J451_RS13100 (J451_13170) pilC 2560556..2561752 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  J451_RS13105 (J451_13175) - 2561755..2562411 (+) 657 WP_006248404.1 prepilin peptidase -
  J451_RS13110 (J451_13180) coaE 2562447..2563082 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  J451_RS13115 (J451_13185) yacG 2563064..2563249 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  J451_RS13120 (J451_13190) holA 2563250..2564275 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  J451_RS13125 (J451_13195) lptE 2564318..2564806 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=59833 J451_RS13090 WP_006248401.1 2559607..2560086(+) (pilA) [Mannheimia haemolytica D174]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=59833 J451_RS13090 WP_006248401.1 2559607..2560086(+) (pilA) [Mannheimia haemolytica D174]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491

Multiple sequence alignment