Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   J450_RS11735 Genome accession   NC_021738
Coordinates   2387213..2388409 (+) Length   398 a.a.
NCBI ID   WP_006248403.1    Uniprot ID   A0A249A0I8
Organism   Mannheimia haemolytica D171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2382213..2393409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J450_RS11710 (J450_11525) folA 2382866..2383360 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  J450_RS11715 (J450_11530) - 2383480..2383710 (+) 231 WP_006251345.1 YgjV family protein -
  J450_RS11720 (J450_11535) radA 2383765..2385144 (-) 1380 WP_006251344.1 DNA repair protein RadA -
  J450_RS11725 (J450_11540) pilA 2385326..2385805 (+) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  J450_RS11730 (J450_11545) pilB 2385826..2387229 (+) 1404 WP_006251341.1 GspE/PulE family protein Machinery gene
  J450_RS11735 (J450_11550) pilC 2387213..2388409 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  J450_RS11740 (J450_11555) - 2388412..2389068 (+) 657 WP_006248404.1 prepilin peptidase -
  J450_RS11745 (J450_11560) coaE 2389104..2389739 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  J450_RS11750 (J450_11565) yacG 2389721..2389906 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  J450_RS11755 (J450_11570) holA 2389907..2390932 (-) 1026 WP_032844853.1 DNA polymerase III subunit delta -
  J450_RS11760 (J450_11575) lptE 2390975..2391463 (-) 489 WP_006251337.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45186.75 Da        Isoelectric Point: 9.4698

>NTDB_id=59812 J450_RS11735 WP_006248403.1 2387213..2388409(+) (pilC) [Mannheimia haemolytica D171]
MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIRISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQA
LSMLLQNCRNIRLYLWLNELIQHIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGTVLLGTLLLAVIFYLFFAKKSRL
VAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLNAHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=59812 J450_RS11735 WP_006248403.1 2387213..2388409(+) (pilC) [Mannheimia haemolytica D171]
ATGACGAAAATCTATGAATTTCATTGGCAAGCTCAGAGCCGATTTGGGCAAAAACAGCGGGGCAAGCGGTTGGCAACGAA
TCGAGACATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCATATTCGGATTAGCCGAAATTTTGTGCTTTCAGCAA
ACCCGAAAAAAGAAGAGGTGAATAGCTTGCTATCACAGCTTGCATTGTTGGTTAATTCTGCCATGCCGTTAAAACAGGCA
TTATCTATGTTGTTGCAAAACTGCCGAAATATCCGCCTTTATCTATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCTTTTTCACAATCGTTGGAGAAATTGGGTGAATTTGTGACCAATCAGGAAATTCAGCTAATCAAAATGGGCGAAC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCGAAATTGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTCTACCCGGTGATTGTGTTGCTGATTTCGCTCGCTTTATCTATCGGGCTATTGGTTTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGCACAGTATTACTTGGCACGTTACTACTAGCGGTCATTTTTTACCTGTTTTTTGCAAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGCCGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAATATTGCCTTAATGCTGAATGCTCATATTCCGCTTGATGTTGCCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGTGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGCAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGCGTGATTGTCGGCACTATTATTGTTGGGCTGTATTTACCGATTTTTGATATGGGGGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0I8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

54

100

0.543
  pilC Haemophilus influenzae Rd KW20

42.145

100

0.425
  pilC Haemophilus influenzae 86-028NP

41.147

100

0.415

Multiple sequence alignment