NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	NAG69_RS03470	Genome accession	NZ_CP097881
Coordinates	713242..713901 (-)	Length	219 a.a.
NCBI ID	WP_001221495.1	Uniprot ID	P52076
Organism	Escherichia coli str. K-12 substr. MG1655 strain K-12
Function	repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 708242..718901

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NAG69_RS03445	parE	708333..710225 (+)	1893	WP_000195296.1	DNA topoisomerase IV subunit B	-
NAG69_RS03450	ygiN	710273..710587 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
NAG69_RS03455	mdaB	710618..711199 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
NAG69_RS03460	ygiZ	711518..711850 (+)	333	WP_000917684.1	DUF2645 family protein	-
NAG69_RS03465	qseC	711896..713245 (-)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
NAG69_RS03470	ciaR	713242..713901 (-)	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	Regulator
NAG69_RS03475	ygiW	714053..714445 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
NAG69_RS03480	ygiV	714498..714980 (+)	483	WP_000183505.1	GyrI-like domain-containing protein	-
NAG69_RS03485	mqsR	715185..715481 (+)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
NAG69_RS03490	mqsA	715483..715878 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
NAG69_RS03495	ygiS	716011..717618 (+)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-

Sequence

Protein

Download Length: 219 a.a. Molecular weight: 24677.59 Da Isoelectric Point: 6.9850

                                >NTDB_id=596846 NAG69_RS03470 WP_001221495.1 713242..713901(-) (ciaR) [Escherichia coli str. K-12 substr. MG1655 strain K-12]

MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA

RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL

LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

Download Length: 660 bp

                                >NTDB_id=596846 NAG69_RS03470 WP_001221495.1 713242..713901(-) (ciaR) [Escherichia coli str. K-12 substr. MG1655 strain K-12]

ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT

CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT

TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG

CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA

AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC

TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA

CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG

TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT

ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus pneumoniae Rx1	37.885	100	0.393
ciaR	Streptococcus pneumoniae D39	37.885	100	0.393
ciaR	Streptococcus pneumoniae R6	37.885	100	0.393
ciaR	Streptococcus pneumoniae TIGR4	37.885	100	0.393
ciaR	Streptococcus mutans UA159	35.426	100	0.361

Detailed information NTG0596846