Detailed information NTG0596734

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M8779_RS10205
• Genome accession: NZ_CP097846
• Coordinates: 1949666..1950892 (-)
• Length: 408 a.a.
• NCBI ID: WP_003697786.1
• Uniprot ID: B4RPS5
• Organism: Neisseria gonorrhoeae strain AT159
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1944666..1955892

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M8779_RS10195 (M8779_10190)	-	1946183..1946467 (+)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
M8779_RS10200 (M8779_10195)	yccS	1947399..1949549 (-)	2151	WP_003697785.1	YccS family putative transporter	-
M8779_RS10205 (M8779_10200)	pilU	1949666..1950892 (-)	1227	WP_003697786.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M8779_RS10210 (M8779_10205)	pilT	1951056..1952099 (-)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
M8779_RS10215 (M8779_10210)	-	1952219..1952905 (+)	687	WP_003699139.1	YggS family pyridoxal phosphate-dependent enzyme	-
M8779_RS10220 (M8779_10215)	-	1952905..1953312 (+)	408	WP_003690202.1	hypothetical protein	-
M8779_RS10225 (M8779_10220)	proC	1953343..1954152 (+)	810	WP_228547131.1	pyrroline-5-carboxylate reductase	-
M8779_RS10230 (M8779_10225)	dksA	1954300..1954716 (+)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
M8779_RS10235 (M8779_10230)	-	1954731..1954892 (+)	162	WP_252295620.1	hypothetical protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45668.30 Da
• Isoelectric Point: 7.2909

>NTDB_id=596734 M8779_RS10205 WP_003697786.1 1949666..1950892(-) (pilU) [Neisseria gonorrhoeae strain AT159]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=596734 M8779_RS10205 WP_003697786.1 1949666..1950892(-) (pilU) [Neisseria gonorrhoeae strain AT159]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAGGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: B4RPS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387