Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABEKA_RS12640 Genome accession   NZ_CP080636
Coordinates   2308812..2310008 (+) Length   398 a.a.
NCBI ID   WP_005266299.1    Uniprot ID   N9QJU4
Organism   Acinetobacter lwoffii strain EK30A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2303812..2315008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEKA_RS12625 - 2305149..2306256 (+) 1108 Protein_2155 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
  ABEKA_RS12630 (ABEKA_2360) - 2306465..2306995 (+) 531 WP_005266297.1 hypothetical protein -
  ABEKA_RS12635 (ABEKA_2361) - 2307517..2308566 (+) 1050 WP_005266298.1 3-deoxy-7-phosphoheptulonate synthase -
  ABEKA_RS12640 (ABEKA_2362) pilU 2308812..2310008 (+) 1197 WP_005266299.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABEKA_RS12645 (ABEKA_2363) gcvH 2310081..2310455 (-) 375 WP_004647068.1 glycine cleavage system protein GcvH -
  ABEKA_RS12650 (ABEKA_2364) - 2310538..2311566 (-) 1029 WP_005246182.1 AraC family transcriptional regulator -
  ABEKA_RS12655 (ABEKA_2365) - 2312018..2313871 (-) 1854 WP_005266300.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45111.56 Da        Isoelectric Point: 6.4476

>NTDB_id=594629 ABEKA_RS12640 WP_005266299.1 2308812..2310008(+) (pilU) [Acinetobacter lwoffii strain EK30A]
MFALELLEEARKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQTLTAEKTKLFAYSLMNEKQRNEFETELEC
NFAITVPGISRFRVNVFQQQLNIGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGYIITVEDPVEYVHKHKKSMITHREVGVDSHSWQNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGKGRRAAIEIMLNTPLISDLILKGEFHELKAIMAKS
RELGMQTFDQALFELYNQGEISYEEALRNADSTNELRLQIKLKSNRQDNAPAGQATQFSMITEPKTTDKDAEDHSETE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=594629 ABEKA_RS12640 WP_005266299.1 2308812..2310008(+) (pilU) [Acinetobacter lwoffii strain EK30A]
ATGTTTGCGCTAGAGCTTTTAGAAGAAGCACGAAAATTTATGCACCATATGCTAACCAAAGTGGTTGAATATGGCGGTTC
AGATTTATTTATTACTGCAGATTTTCCACCAAGTATTAAACATCAAGGTTTGATGAAACCATTTGGACAGCAGACCCTGA
CCGCTGAGAAAACCAAGCTCTTTGCTTATAGCCTAATGAATGAAAAACAGCGCAATGAATTTGAAACCGAACTTGAGTGT
AATTTTGCGATTACTGTTCCGGGTATTTCACGTTTCCGGGTCAATGTATTCCAGCAACAGTTGAATATCGGTATGGTGAT
TCGTACCATCACTGCGGAAATCCCAAATTTCCAGAAGTTAAAACTGCCTGAATCGCTGAAACATGTGATTATGGAAAAAC
GTGGTCTAGTCTTGGTGGTCGGTGGAACCGGTTCGGGTAAATCAACTTCACTGGCAGCGATGATTGACTATCGGAATGAA
AACTCGGCTGGATATATCATCACGGTAGAAGATCCGGTGGAATATGTACACAAGCATAAAAAATCCATGATTACCCATCG
TGAAGTCGGGGTAGATAGCCATTCTTGGCAAAATGCTTTAAAAAATACCTTACGTCAGGCACCCGATGTGATTTTGATCG
GGGAAATCCGCGATACAGAAACTATGGAACATGCGATTGCTTTTGCTGAAACTGGGCATCTTTGTTTAGGTACGTTGCAT
TCCAATAATGCCAACCAGACTTTAGACCGGATTATTAACTTCTTCCCAGAAGAACGTCGCAATCAGTTGTTGATGGATTT
GTCCTCCAATATGAAGGCTATTATTTCACAGCGTTTGGTTCGGACTCAGGATGGCAAGGGACGTCGAGCAGCCATTGAAA
TCATGTTAAATACACCGCTCATTTCAGACTTGATTTTGAAAGGAGAGTTTCATGAGTTGAAAGCGATTATGGCAAAATCA
CGGGAATTGGGAATGCAGACCTTTGATCAGGCTTTATTTGAACTCTATAACCAAGGCGAAATTTCTTATGAAGAAGCATT
ACGTAATGCCGATTCAACCAATGAATTACGTTTACAGATCAAATTAAAAAGTAATCGTCAAGACAATGCTCCGGCTGGGC
AAGCCACCCAATTTTCCATGATTACAGAACCGAAAACAACTGATAAAGATGCAGAAGATCATTCGGAAACGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9QJU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.069

87.437

0.543
  pilU Acinetobacter baylyi ADP1

52.815

93.719

0.495
  pilU Vibrio cholerae strain A1552

51.507

91.709

0.472