Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   M7749_RS03545 Genome accession   NZ_CP097360
Coordinates   719643..720302 (-) Length   219 a.a.
NCBI ID   WP_001546136.1    Uniprot ID   -
Organism   Escherichia coli strain AGR4587     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 714643..725302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M7749_RS03515 (M7749_03515) ygiN 715544..715858 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  M7749_RS03520 (M7749_03520) mdaB 715889..716470 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  M7749_RS03525 (M7749_03525) - 716720..717199 (+) 480 WP_000065331.1 Hcp family type VI secretion system effector -
  M7749_RS03530 (M7749_03530) - 717202..717912 (+) 711 WP_000834022.1 hypothetical protein -
  M7749_RS03535 (M7749_03535) ygiZ 717919..718251 (+) 333 WP_000914696.1 DUF2645 family protein -
  M7749_RS03540 (M7749_03540) qseC 718297..719646 (-) 1350 WP_001546137.1 quorum sensing histidine kinase QseC -
  M7749_RS03545 (M7749_03545) ciaR 719643..720302 (-) 660 WP_001546136.1 quorum sensing response regulator transcription factor QseB Regulator
  M7749_RS03550 (M7749_03550) ygiW 720454..720846 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  M7749_RS03555 (M7749_03555) ygiV 720899..721381 (+) 483 WP_000183493.1 GyrI-like domain-containing protein -
  M7749_RS03560 (M7749_03560) ygiS 721490..723097 (+) 1608 WP_001468194.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24615.56 Da        Isoelectric Point: 7.4216

>NTDB_id=594435 M7749_RS03545 WP_001546136.1 719643..720302(-) (ciaR) [Escherichia coli strain AGR4587]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWRGKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=594435 M7749_RS03545 WP_001546136.1 719643..720302(-) (ciaR) [Escherichia coli strain AGR4587]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAAGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGGAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361