Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   K0V03_RS01895 Genome accession   NZ_CP080508
Coordinates   325984..326580 (-) Length   198 a.a.
NCBI ID   WP_085186512.1    Uniprot ID   -
Organism   Bacillus subtilis strain HD15     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320984..331580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0V03_RS01865 (K0V03_01865) rpsT 321286..321552 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  K0V03_RS01870 (K0V03_01870) holA 321567..322610 (-) 1044 WP_021480061.1 DNA polymerase III subunit delta -
  K0V03_RS01875 (K0V03_01875) - 322650..322763 (-) 114 WP_122060479.1 hypothetical protein -
  K0V03_RS01880 (K0V03_01880) yqzM 322840..322974 (+) 135 WP_003229983.1 YqzM family protein -
  K0V03_RS01885 (K0V03_01885) - 323015..325224 (-) 2210 Protein_374 DNA internalization-related competence protein ComEC/Rec2 -
  K0V03_RS01890 (K0V03_01890) comEB 325348..325917 (-) 570 WP_072174291.1 ComE operon protein 2 -
  K0V03_RS01895 (K0V03_01895) comEA 325984..326580 (-) 597 WP_085186512.1 competence protein ComEA Machinery gene
  K0V03_RS01900 (K0V03_01900) comER 326664..327485 (+) 822 WP_004398597.1 late competence protein ComER -
  K0V03_RS01905 (K0V03_01905) yqeM 327551..328294 (-) 744 WP_070547553.1 class I SAM-dependent methyltransferase -
  K0V03_RS01910 (K0V03_01910) rsfS 328291..328647 (-) 357 WP_014480315.1 ribosome silencing factor -
  K0V03_RS01915 (K0V03_01915) yqeK 328665..329225 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  K0V03_RS01920 (K0V03_01920) nadD 329215..329784 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  K0V03_RS01925 (K0V03_01925) yhbY 329796..330086 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  K0V03_RS01930 (K0V03_01930) aroE 330080..330922 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 21180.92 Da        Isoelectric Point: 4.8417

>NTDB_id=593294 K0V03_RS01895 WP_085186512.1 325984..326580(-) (comEA) [Bacillus subtilis strain HD15]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGDGGKGALVNINTATLEELQGISGVGPSKAEA
IIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=593294 K0V03_RS01895 WP_085186512.1 325984..326580(-) (comEA) [Bacillus subtilis strain HD15]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTACAGCAAGGCGACGGAGGGAAGG
GAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTGGGGCCATCCAAAGCTGAAGCT
ATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGTTTCAGGAATAGGTGAAAAGTC
ATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(135-196)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

94.146

100

0.975
  comEA Staphylococcus aureus MW2

34.389

100

0.384
  comEA Staphylococcus aureus N315

33.937

100

0.379