Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   M2J01_RS28640 Genome accession   NZ_CP096964
Coordinates   6159097..6159720 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain NY13936     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6154097..6164720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J01_RS28620 (M2J01_28560) aroB 6154692..6155798 (-) 1107 WP_058161056.1 3-dehydroquinate synthase -
  M2J01_RS28625 (M2J01_28565) aroK 6155848..6156366 (-) 519 WP_003095833.1 shikimate kinase AroK -
  M2J01_RS28630 (M2J01_28570) pilQ 6156378..6158522 (-) 2145 WP_058161055.1 type 4a pilus secretin PilQ Machinery gene
  M2J01_RS28635 (M2J01_28575) comL/pilP 6158576..6159100 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  M2J01_RS28640 (M2J01_28580) pilO 6159097..6159720 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  M2J01_RS28645 (M2J01_28585) pilN 6159717..6160313 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  M2J01_RS28650 (M2J01_28590) pilM 6160313..6161377 (-) 1065 WP_058161054.1 type IV pilus assembly protein PilM Machinery gene
  M2J01_RS28655 (M2J01_28595) - 6161562..6164033 (+) 2472 WP_023126737.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=593145 M2J01_RS28640 WP_003103268.1 6159097..6159720(-) (pilO) [Pseudomonas aeruginosa strain NY13936]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=593145 M2J01_RS28640 WP_003103268.1 6159097..6159720(-) (pilO) [Pseudomonas aeruginosa strain NY13936]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTTGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401