Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   KXE03_RS23635 Genome accession   NZ_CP080007
Coordinates   5142760..5144352 (+) Length   530 a.a.
NCBI ID   WP_058166740.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain S-1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5137760..5149352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KXE03_RS23615 (KXE03_23605) pgeF 5139510..5140238 (-) 729 WP_023435616.1 peptidoglycan editing factor PgeF -
  KXE03_RS23620 (KXE03_23610) rluD 5140235..5141197 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  KXE03_RS23625 (KXE03_23615) - 5141343..5142368 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  KXE03_RS23630 (KXE03_23620) - 5142525..5142770 (+) 246 WP_003102591.1 PP0621 family protein -
  KXE03_RS23635 (KXE03_23625) pilS 5142760..5144352 (+) 1593 WP_058166740.1 two-component system sensor histidine kinase PilS Regulator
  KXE03_RS23640 (KXE03_23630) pilR 5144367..5145704 (+) 1338 WP_034014849.1 two-component system response regulator PilR Regulator
  KXE03_RS23645 (KXE03_23635) thiO 5145750..5146844 (-) 1095 WP_074235043.1 glycine oxidase ThiO -
  KXE03_RS23650 (KXE03_23640) - 5146984..5147484 (+) 501 WP_016254062.1 GspH/FimT family pseudopilin -
  KXE03_RS23655 (KXE03_23645) - 5147607..5148095 (+) 489 WP_003141552.1 GspH/FimT family pseudopilin -
  KXE03_RS23660 (KXE03_23650) pilV 5148105..5148629 (+) 525 WP_003116261.1 type IV pilus modification protein PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59016.17 Da        Isoelectric Point: 6.5650

>NTDB_id=590718 KXE03_RS23635 WP_058166740.1 5142760..5144352(+) (pilS) [Pseudomonas aeruginosa strain S-1]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRSQVWLSLARDPESDLPVLEVIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=590718 KXE03_RS23635 WP_058166740.1 5142760..5144352(+) (pilS) [Pseudomonas aeruginosa strain S-1]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGTAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGACGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
GCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATATCCCGGCAGGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCAGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGGCGGACAAATTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.623

100

0.996