NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	KXY09_RS13075	Genome accession	NZ_CP079719
Coordinates	2684328..2684942 (-)	Length	204 a.a.
NCBI ID	WP_015240245.1	Uniprot ID	-
Organism	Bacillus velezensis strain LABIM44
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2679328..2689942

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KXY09_RS13045 (KXY09_13040)	rpsT	2679631..2679897 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
KXY09_RS13050 (KXY09_13045)	holA	2679914..2680955 (-)	1042	Protein_2549	DNA polymerase III subunit delta	-
KXY09_RS13055 (KXY09_13050)	-	2680995..2681146 (-)	152	Protein_2550	hypothetical protein	-
KXY09_RS13060 (KXY09_13055)	-	2681187..2681321 (+)	135	WP_003152870.1	YqzM family protein	-
KXY09_RS13065 (KXY09_13060)	comEC	2681340..2683691 (-)	2352	WP_168985061.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KXY09_RS13070 (KXY09_13065)	-	2683692..2684261 (-)	570	WP_003152868.1	ComE operon protein 2	-
KXY09_RS13075 (KXY09_13070)	comEA	2684328..2684942 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
KXY09_RS13080 (KXY09_13075)	comER	2685001..2685822 (+)	822	WP_168985062.1	late competence protein ComER	-
KXY09_RS13085 (KXY09_13080)	-	2685891..2686628 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
KXY09_RS13090 (KXY09_13085)	rsfS	2686625..2686981 (-)	357	WP_007408260.1	ribosome silencing factor	-
KXY09_RS13095 (KXY09_13090)	yqeK	2686999..2687559 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KXY09_RS13100 (KXY09_13095)	-	2687549..2688118 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
KXY09_RS13105 (KXY09_13100)	yhbY	2688129..2688419 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
KXY09_RS13110 (KXY09_13105)	aroE	2688413..2689255 (-)	843	WP_168985063.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22209.32 Da Isoelectric Point: 5.5211

                                >NTDB_id=588919 KXY09_RS13075 WP_015240245.1 2684328..2684942(-) (comEA) [Bacillus velezensis strain LABIM44]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=588919 KXY09_RS13075 WP_015240245.1 2684328..2684942(-) (comEA) [Bacillus velezensis strain LABIM44]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0588919